中国组织工程研究2016，Vol.20Issue(40)：6041-6047,7.DOI:10.3969/j.issn.2095-4344.2016.40.017
2型糖尿病大鼠骨质疏松模型的建立
Establishing a rat model of type 2 diabetes:its bone metabolism level
摘要
Abstract
BACKGROUND:Diabetes mel itus can give rise to bone metabolic disorders that may involve long-term hyperglycemia, hypoglycemic agents, diet control, estrogen, insulin-like growth factor, leptin, body mass, sex and age. <br> OBJECTIVE:To establish type 2 diabetic rat models, and to explore the influence of type 2 diabetes on bone metabolism. <br> METHODS:High-fat and high-glucose diets combining with 35 mg/kg streptozotocin were used to induce type 2 diabetic model in seven male Sprague-Dawley rats (diabetic group). Thirteen rats in control group were given intraperitoneal injection of the same amount of citric acid and sodium citrate buffer. At 4 weeks after modeling, the bone density of rats was serum detected by dual-energy X-ray, levels of fasting blood-glucose, cholesterol, triacylglycerol, serum calcium, phosphate, alkaline phosphatase, fasting insulin, osteocalcin and C-terminal telopeptide-I were measured, and morphology of bone was observed. <br> RESULTS AND CONCLUSION:Compared with control group, (1) the rat body mass and fasting blood-glucose kept on an overt rise in the diabetic group (P<0.05);(2) levels of cholesterol, triacylglycerol, fasting insulin and alkaline phosphatase, as wel as insulin resistance index were significantly increased, but insulin sensitivity index was significantly decreased in the diabetic group (P<0.05). (3) There were no significant differences in the levels of serum calcium, phosphate, alkaline phosphatase, osteocalcin and C-terminal telopeptide-I between two groups (P>0.05). (4) In the diabetic group, thinner and sparse bone trabeculae were split presenting more free broken ends;(5) the bone density in lumbar spine, double femoral, pelvic and thoracolumbar spine were al significantly decreased (P<0.05). (6) In conclusion, the type 2 diabetic rat model can be successful y induced by 5-week feeding high-fat and high-glucose diets combining with intraperitoneal injection of 35 mg/kg streptozotocin;these mode rats hold some characters, such as hyperglycemia, dyslipidemia, insulin resistance, diminished bone density, and accelerated bone resorption.关键词
实验动物/内分泌系统损伤与修复动物模型/2型糖尿病/骨质疏松/骨密度/骨形态Key words
Diabetes Mel itus, Type 2/Osteoporosis/Bone Density/Tissue Engineering分类
医药卫生引用本文复制引用
张燕,杨秋萍,赵燕,赵豫梅,谭洪,杜思成..2型糖尿病大鼠骨质疏松模型的建立[J].中国组织工程研究,2016,20(40):6041-6047,7.基金项目
云南省科技厅-昆明医科大学应用基础研究联合专项(2013FZ270)；昆明医科大学研究生创新基金(2014N04)Funding:the Joint Special for Applied Basic Research of Yunnan Provincial Science and Technology Department-Kunming Medical University, No.2013FZ270 （2013FZ270）
the Innovation Foundation for the Postgraduate in Kunming Medical University, No.2014N04 （）
