中国水稻科学2016，Vol.30Issue(5)：447-457,11.DOI:10.16819/j.1001-7216.2016.6048
水稻粉质胚乳突变体 ws 的表型分析及基因克隆
Phenotyping and Gene Cloning of a Floury Endosperm Mutant ws in Rice
摘要
Abstract
In this study,we obtained a stably inherited floury endosperm mutant ws from j aponica variety cv. Dianjingyou 1 (DJY),with its 1000-grain weight,grain size,total starch content,and amylose content in mature seeds all decreased compared with wild-type,as well as the swelling power of starch.By observing the structure of mature and developing endosperm,we found that the starch granules in ws mutant were smaller and loosely packed.The WS locus was first mapped to a region near the centromere in chromosome 8 with 92 recessive individuals,and then was narrowed down to a 95 kb region by using 2025 recessive individuals.Sequence analysis revealed that the coding region of the small subunit of adenosine diphosphate glucose pyrophosphorylase (AGPS2 ) had a single nucleotide substitution,resulting in a change in the amino acid sequence.RT-PCR analysis showed no significant difference in the expression levels of genes encoding the AGPase subunits in the mutant endosperm,while immunoblot analysis revealed a reduced protein content of the AGPS2b.Meanwhile,the enzyme activity of AGPase in the mutant was decreased to half of the wild-type.These results showed that the mutation of OsAGPS2 caused the decreased activity of AGPase, thus affecting the starch biosynthesis in rice endosperm.关键词
胚乳粉质突变体/AGPase/淀粉/基因表达/理化性质/水稻Key words
floury endosperm mutant/AGPase/starch/gene expression/physicochemical property/rice (Oryza sativa L.)分类
生物科学引用本文复制引用
潘鹏屹,朱建平,王云龙,郝媛媛,蔡跃,张文伟,江玲,王益华,万建民..水稻粉质胚乳突变体 ws 的表型分析及基因克隆[J].中国水稻科学,2016,30(5):447-457,11.基金项目
国家转基因生物新品种培育重大专项(2014ZX08001006) （2014ZX08001006）
国家自然科学基金资助项目(31371598) （31371598）
江苏省科技支撑计划资助项目(BE2014394) （BE2014394）
农业部长江中下游粳稻生物学与遗传育种重点实验室、长江流域杂交水稻协同创新中心、江苏省现代作物生产中心资助项目 （）
