中国水稻科学2016，Vol.30Issue(5)：458-468,11.DOI:10.16819/j.1001-7216.2016.5180
干旱胁迫下杂草稻和栽培稻根系基因表达差异研究
Global Genome Expression Analysis of Root Genes under Drought Stress in Weedy Rice and Up-land Rice
摘要
Abstract
The expression changes of root genes in drought-resistance weedy rice HEB07-2 (Oryza sativa f.spontanea ) and up-land rice IAPAR9 (Oryza sativa )were analyzed under polyethylene glycol(PEG)-simulated with drought stress condition with Affymetrix GeneChip rice genome array.The results indicated that the extent and direction of transcriptome response of HEB07-2 and IAPAR9 to drought differed greatly.For HEB07-2,among 6878 expressed genes,4693 were up-regulated and 2185 were down-regulated under drought condition.For IAPAR9,among 2923 expressed genes,983 were up-regulated and 1 940 were down-regulated.Analysis of differentially expressed genes in HEB07-2 and IAPAR9 showed that the weedy rice HEB07-2 had a higher changing fold than the up-land rice IAPAR9. Gene ontology analysis revealed that genes of HEB07-2 in potassium ion transporting (GO:000681 3 ),secondary metabolite(GO:001 9748 ),cell growth (GO:001 6049 ),glucose metabolism (GO:0006006 ),transmembrane ion transporter activity(GO:001 5075 ),ferroheme coalition(GO:0020037 ),oxidordeuctase activity(GO:001 649 1 )were significantly up-regulated in comparing with IAPAR9.These were consistent with the physiological and phenotypic data.关键词
杂草稻/根系/表达谱芯片/耐旱Key words
weedy rice/root/gene expression profiling/drought resistance分类
生物科学引用本文复制引用
丁国华,夏天舒,沈希宏,马殿荣,陈温福,孙健,杨光,张凤鸣,白良明,孙世臣,姜树坤,王彤彤,郑洪亮..干旱胁迫下杂草稻和栽培稻根系基因表达差异研究[J].中国水稻科学,2016,30(5):458-468,11.基金项目
黑龙江省农业科学院博士后工作站 （）
哈尔滨市科技局项目(2014RFQYJ125) （2014RFQYJ125）
黑龙江省农业科学院创新工程项目(2014QN006) （2014QN006）
黑龙江省农业科学院博士人员科研启动金项目(201507-16) （201507-16）
国家水稻产业技术体系项目(CARS-01-43) （CARS-01-43）
北方寒地粳稻资源研究与新品种选育项目(2014BAD01B03-02) （2014BAD01B03-02）
