中国水稻科学2016，Vol.30Issue(5)：479-486,8.DOI:10.16819/j.1001-7216.2016.6037
水稻矮秆多分蘖突变体 mz3的遗传分析和基因定位
Genetic Analysis and Gene Mapping of a Dwarf and High-tillering Mutant mz3 in Rice (Oryza sativa L.)
摘要
Abstract
A dwarf and high-tillering mutant,temporarily termed as mz3 was obtained from Zhonghua 1 1 ,a j aponica rice variety by EMS mutagenesis.Genetic analysis indicated that the mutant phenotype was controlled by a recessive gene.An F2 population was developed by crossing mz3 with Nanjing 1 1 ,an indica rice variety for mapping of the mutant gene,which was ultimately restricted within a physical distance of about 747 kb between two SSR markers RM1 9353 and RM5 10 on the long arm of rice chromosome 6.Gene prediction revealed that D3 ,a gene conferring rice plant height and tiller number was located in this region.Sequence analysis identified a single G to A nuclear acid substitution at the position 636 from the ATG start codon,forming a premature translational termination codon. Sequencing of 10 randomly selected recessive plants from mz3/Nanjing 1 1 mapping population confirmed the same mutation site carried by the 10 individuals.Subcelluar location indicated that D3 protein of the mutant was located in the nuclei as wild type.BiFC analysis showed that D3 protein of the mutant could not interact with D14 protein for lacking of essential amino acids that bind with D14,blocking signal transduction of strigolactones.So we speculated that the mutant phenotype of mz3 was probably caused by mutation in D3 gene.关键词
水稻/矮秆多分蘖/D3/基因定位Key words
rice/dwarf and high-tillering/D3/gene mapping分类
生物科学引用本文复制引用
苏晓妹,方宇星,刘钰龙,刘峰,张所兵,张云辉,鲍依群..水稻矮秆多分蘖突变体 mz3的遗传分析和基因定位[J].中国水稻科学,2016,30(5):479-486,8.基金项目
国家自然科学基金资助项目(31401036) （31401036）
江苏省自然科学基金资助项目(BK20130706) （BK20130706）
江苏省农业科技自主创新资金资助项目[(CX(14)5005)] （CX(14）
江苏省农业科学院基本科研业务专项[(ZX(15)4015)] （ZX(15）
