中国水稻科学2016，Vol.30Issue(5)：487-492,6.DOI:10.16819/j.1001-7216.2016.6038
水稻粒宽基因 GS5的功能标记开发和单倍型鉴定
Development of Functional Markers and Identification of Haplotypes for Rice Grain Width Gene GS5
摘要
Abstract
As a major component of rice grain shape,the grain width is an important trait which is correlated with the rice yield and quality.Based on the alignment of gene GS5 genomic DNA sequence,two functional markers were developed based on two missense polymorphisms (ACC/CTA in the exon2, A/C in the exon9, respectively ). Subsequently,the markers were used to identify the genotypes of gene GS5 in the 294 accessions of a rice mini-core collection and 65 j aponica varieties released in Jiangsu Province from 2007 to 201 3.We found that the allelic variations at the two target loci had significant or extremely significant differences in the grain shape traits(grain length,grain width and ratio of length to width).Based on the two variation sites of gene GS5 ,four haplotypes (combinations)were found in the rice mini-core collection,and had extremely significant effect on grain width,grain thickness and ratio of length to width in the indica group,and on grain width and ratio of length to width in the j aponica group, respectively.While in j aponica varieties released by Jiangsu,only two haplotypes,Hap1 (represented by 64 varieties) and Hap2 (represented by one variety)were found.The results will lay a foundation for the application of the useful al-lelic variations or haplotypes of gene GS5 to the rice yield and quality breeding program.关键词
水稻/粒形/基因 GS5/功能标记/单倍型Key words
rice/grain shape/gene GS5/functional markers/haplotype分类
生物科学引用本文复制引用
裔传灯,王德荣,蒋伟,李玮,成晓俊,王颖,周勇,梁国华,顾铭洪..水稻粒宽基因 GS5的功能标记开发和单倍型鉴定[J].中国水稻科学,2016,30(5):487-492,6.基金项目
国家自然科学基金资助项目(31571624,31071382) （31571624,31071382）
国家重点基础研究发展计划资助项目(2010CB125904,2013CBA01405) （2010CB125904,2013CBA01405）
江苏省高校自然科学研究重大项目(15KJA210004) （15KJA210004）
江苏省重点研发计划资助项目(BE2015341) （BE2015341）
江苏高校优势学科建设工程资助项目 （）
