中国水稻科学2016，Vol.30Issue(5)：541-551,11.DOI:10.16819/j.1001-7216.2016.5198
稻曲病菌 T-DNA 插入突变体 B2510的插入位点分析
Molecular Characterization of Ustilaginoidea virens T-DNA Insertion Mutant B2510
摘要
Abstract
To isolate the gene concerned with molecular pathogenic process of Ustilaginoidea virens (U .virens ), T-DNA integration flanking sequence and the mutant genes of a mutant strain B25 10 were analyzed.Compared with the wild-type U .virens strain P1 ,the pathogenicity of the mutant strain B25 10 in the field was significantly decreased.The growth rate of B25 10 on MM medium was slower than that of P1 ,but was not significantly different on PSA and TB3 medium which were nutritionally endowed.The mutant strain B25 10 did not produce conidiophores in PS broth medium.Genomic Southern bolt analysis confirmed that there were double T-DNA events inserted in genome of mutant strain B25 10.The flanking U .virens sequences of T-DNA obtained by TAIL-PCR were adjacent in the wild type and with no sequences lost,and only a few bases of T-DNA were changed.The T-DNA insertion site was in the promoter region of UV8b _ 1412 and downstream 3′region of UV8b _ 1386 ,respectively.RT-PCR analysis confirmed that the expression of both of two genes in B25 10 were significantly decreased.The genes which were affected by T-DNA insertion may be associated with pathogenicity and participate in the regulation of pathogenic process of U .virens in rice.关键词
稻曲病菌/T-DNA 插入突变/ATMT 转化/致病力/侧翼序列Key words
Ustilaginoidea virens/T-DNA insertion mutant/ATMT transformation/pathogenicity/flanking sequence分类
生物科学引用本文复制引用
丁慧,俞咪娜,王亚会,于俊杰,尹小乐,薄惠文,黄星,刘永锋..稻曲病菌 T-DNA 插入突变体 B2510的插入位点分析[J].中国水稻科学,2016,30(5):541-551,11.基金项目
江苏省农业科技自主创新基金资助项目[CX(15)1054] （15）
国家自然科学基金资助项目(31301624) （31301624）
