中国实验动物学报2016，Vol.24Issue(4)：333-338,6.DOI:10.3969/j.issn.1005-4847.2016.04.001
Pin1在皮肤组织中的表达以及可诱导转基因小鼠模型的建立
Pin1 expression in the skin and establishment of an inducible transgenic mouse model
摘要
Abstract
Objective To observe the Pin1 expression pattern in skin and to establish an inducible skin specific Pin1 overexpression mouse model. Methods The mouse Pin1 gene was cloned into modified vector pTRE2 with C⁃terminal Myc tag. The linearized pTRE2⁃Pin1 DNA was micro⁃injected into one⁃cell embryos followed by implantation into foster mice to produce TRE⁃Pin1 transgenic mice. Results TRE⁃Pin1 transgenic founder mice were successfully created. These mice were crossed with transgenic tool mice K14⁃rtTA to create epithelial specific double transgenic progenies. Pin1 gene was induced by incorporating doxycycline into drinking water of the mice. Pin1 protein overexpression in the skin was con⁃firmed by Western blot and immunohistochemistry. The endogenous Pin1 protein was predominantly expressed in epidermal cells in the skin. Conclusions The inducible skin specific Pin1 overexpression mouse model is successfully established which may serve as a useful model for further study of Pin1 functions in the skin.关键词
Pin1/多西霉素/转基因小鼠/Tet-on/皮肤Key words
Pin1/Doxycycline/Transgenic mice/Tet-on/Skin分类
生物科学引用本文复制引用
向建,陈鹏,张莉,卢坤平,廖新化..Pin1在皮肤组织中的表达以及可诱导转基因小鼠模型的建立[J].中国实验动物学报,2016,24(4):333-338,6.基金项目
国家自然科学基金（编号81573029）；福建省自然科学基金（编号2015J01312）；福建省2011计划干细胞转化医学协同创新中心。 （）
