中国实验动物学报2016，Vol.24Issue(4)：375-380,6.DOI:10.3969/j.issn.1005-4847.2016.04.008
GGTA1－／－五指山小型猪SLA I类基因分子特征及与HLA相似性分析
Characterization of swine leukocyte antigen class I genes and homology analysis of the similarity to HLA in GGTA1 -/- Wuzhishan minipigs
摘要
Abstract
Objective This study was aimed to characterize the swine leukocyte antigen( SLA) class I genes of GGTA1 -/ - Wuzhishan minipigs and compare their similarity to human leukocyte antigen( HLA) . It has important implica⁃tions for understanding the cellular rejection in xenotransplantation. Methods Specimens of ear tissue from six founding GGTA1 -/ - Wuzhishan minipigs were collected, and the SLA class I genes (SLA⁃1, SLA⁃3, SLA⁃2) were amplified by RT⁃PCR. Purified products were cloned into pEASY⁃T1 vectors and sequenced, followed by BLAST alignment and using bioin⁃ formatc analysis to characterize the SLA class I genes and compare with the similarity to HLA. Results A total of six al⁃leles were detected, among them alleles were previously reported (SLA⁃1∗0703,SLA⁃2∗1102, SLA⁃3∗0401, SLA⁃3∗0403), and the other were novel (SLA⁃1∗0401wz01, SLA⁃2∗11wz01). The homology between alleles of SLA class I genes in Wuzhishan minipigs and HLA was from 70�5% to 72�1%. The homology analysis of critical amino acid residues on HLA binding with human CD8 + molecules showed that SLA⁃1∗0401wz01, SLA⁃1∗0703, SLA⁃2∗11wz01, SLA⁃2∗1102 and SLA⁃3∗0401 occurred mutant at amino acid positions 225 and 228 ( T→S,T→M) , whereas the other loci were highly conserved. There was a high homology at amino acid level between SLA⁃2∗11wz01, SLA⁃2∗1102 and HLA class I genes which are NK cell KIRs binding sites. Conclusions The amino acid sequences of SLA class I genes of GGTA1 -/ -Wuzhishan minipigs have a high homology to HLA. From the point of view of cell⁃mediated xenograft rejection, the amino acid sequences of SLA class I genes of GGTA1 -/ - Wuzhishan minipigs have a high homology to HLA, therefore, Wzhishan minipigs may become a good potential donor for pig⁃human xenotransplantation.关键词
GGTA1 -/-/五指山小型猪/SLA/异种移植/免疫排斥Key words
GGTA1-/ -/Wuzhishan minipigs/Swine leukocyte antigen, SLA/Xenotransplantation/Immune rejection分类
生物科学引用本文复制引用
蒋应弟,曾国敏,石宁宁,李西睿,纪慧丽,潘登科,滚双宝..GGTA1－／－五指山小型猪SLA I类基因分子特征及与HLA相似性分析[J].中国实验动物学报,2016,24(4):375-380,6.基金项目
国家重点基础研究发展计划（编号2015CB554103）。 （）
