中国比较医学杂志2016，Vol.26Issue(8)：42-46,5.DOI:10.3969/j.issn.1671-7856.2016.08.006
小鼠尾静脉注射与心肌注射腺病毒载体转染效率的比较
Comparison of the efficiency of tail vein injection and intramyocardial injection of adenovirus vector in mice
摘要
Abstract
Objective To investigate the efficiency of target gene transfection of the heart and liver after tail vein or intramyocardial injection of adenovirus vector (GFP-Ad).Methods GFP-AD was constructed at first.A total of 20 male 8-week old C57BL/6 mice were randomly and equally divided into tail vein injection of GFP-AD group and intramyocardial injection of GFP-AD group.The mRNA levels of GFP in the heart and liver tissues were detected by Q-PCR at different time points.Fluorescence microscopy was performed to visualize the expression of GFP fluorescence.Results Compared with the tail vein injection group, the GFP mRNA level in mouse heart tissue was apparently higher in the intramyocardial injection group.In both groups, the GFP mRNA levels in liver tissue were significantly increased compared with that in the heart tissue.In the tail vein injection group, the GFP mRNA level in liver tissue reached a peak on day 7;but in the intramyocardial injection group, the mRNA level of GFP in liver tissue reached apeak on day 3.We also observed the same trend of GFP fluorescence expression in the tail vein injection group compared with that in the intramyocardial injection group.Conclusions Intramyocardial injection of adenovirus vector is suitable to achieve a higher transfection efficiency in mouse heart tissue compared with the tail vein injection method.Although both injection methods are suitable for transfection of mouse liver, the tail vein injection method is preferential for it is simple and less invasive.关键词
心肌注射/尾静脉注射/腺病毒载体/转染效率Key words
Intramyocardial injection/Tail vein injection/Adenovirus vector/Transfection efficiency,Mice分类
医药卫生引用本文复制引用
杨琳珊,占贞贞,汪波,杨珺琦,范慧敏,刘中民..小鼠尾静脉注射与心肌注射腺病毒载体转染效率的比较[J].中国比较医学杂志,2016,26(8):42-46,5.基金项目
国家自然科学基金面上项目（81370433，81373146，81571541）；上海市“创新行动计划”基础研究领域项目（14JC1405200）。 （）
