中国比较医学杂志2016，Vol.26Issue(8)：85-90,6.DOI:10.3969/j.issn.1671-7856.2016.08.014
利用吗啉代寡核苷酸技术下调早期斑马鱼胚胎lmna 基因的初步研究
Study on the lmna gene knockdown in zebrafish embryo with morpholino oligonucleotides
摘要
Abstract
Objective Lamins are the major components of nuclear lamina underneath the inner nuclear membrane (INM).Lamins express in most cells and are involved in the whole process of growth, also play a major role in cell stability and embryonic development.Mutant in human LMNA gene may lead to a series of disorders, which are similar to progeria or other aging-associate syndrome.In this study, we report a new lmna knockdown animal model generated in our laboratory in order to provide a useful tool for studying laminopathies.Methods Two plasmids tagged to zebrafish lmna gene were designed based on morpholino oligonucleotides technology.Co-microinjected the plasmids into zebrafish embryos to knockdown lmna gene.Imagining and western blot detection were used to identify the mutants.Results Two different proteins, Lamin A/C, were expressed in the zebrafish embryos.Two plasmids lmna-MO and lmna-EGFP-pCS 2 + were generated and co-microinjected into embryos.The results of imagining and western blot showed that the expression of lmna gene was downregulated in the zebrafish embryos.Conclusions Lamin A/C are expressed in zebrafish.lmna gene can be knocked down by the injection of lmna-MO and lmna-EGFP-pCS 2 +.This new animal model may be a powerful tool for study on laminopathies.关键词
斑马鱼/lmna/基因下调/吗啉代寡核苷酸技术Key words
Zebrafish/lmna/Gene knockdown/Morpholino oligonucleotides/Laminopathy分类
医药卫生引用本文复制引用
刘丰,黄慧敏,王志华,吴西军,何志旭,舒莉萍..利用吗啉代寡核苷酸技术下调早期斑马鱼胚胎lmna 基因的初步研究[J].中国比较医学杂志,2016,26(8):85-90,6.基金项目
国家自然科学基金项目资助（NO．31260284）。 （）
