中国比较医学杂志2016，Vol.26Issue(9)：7-12,6.DOI:10.3969/j.issn.1671-7856.2016.09.002
hBMSCs幼年食蟹猴脑内移植的安全评价、迁移及分布
Safty evaluation, migration and distribution of human bone marrow derived-mesenchymal stem cells in the cns of young macaca fascicularis
摘要
Abstract
Objective To evalutate the safty of hBMSCs transpalntation and to observe their migration and distribution in the brain of young macaca fascicularis. To establish a new technology platform and theoretical basis for the treatment of central nervous system diseases in children. Methods Labelled hBMSCs were transplanted into the striatum of young macaca fascicularis. Brain sections were examined to evalutate the inflammatory reaction and immunological rejection of local injection sites by HE observation and immunohistochemical staining. Migration and distribution of transplanted⁃hBMSCs was observed by real⁃time fluorescence quantitative PCR of male DNA and fluorescence microscope. Results The results showed that the direct intracerebral injection of hBMSCs did not cause systemic symptoms in animals. There is no inflammatory reaction and immunological rejection was detected, and degeneration and necrosis of neural cells and proliferation of glial cells were absent in the local injection sites. The transplanted hBMSCs survived, and migrated into the brain after 4 weeks transplantation. Its migration and distribution have certain regularity and were overlapping between transplant recipients. In addtion, hBMSCs tended to extend rostrally into the forebrain and showed preference of migrating toward the blood vessels and below the ependyma. Conculsions Intracerebral transplantation of hBMSCs is safe. And hBMSCs can survive and migrate into the brain.关键词
人骨髓间充质干细胞/食蟹猴/迁移/分布Key words
Human bone marrow derived-mesenchymal stem cells/Macaca fascicularis/Migration/Distribution分类
医药卫生引用本文复制引用
李加美,朱华,姚志刚,邓威,李秦,马春梅,秦川..hBMSCs幼年食蟹猴脑内移植的安全评价、迁移及分布[J].中国比较医学杂志,2016,26(9):7-12,6.基金项目
国家自然科学基金项目（81502568，81402479），山东省医药科技发展计划项目（2014WS0355）。 （）
