中国现代医学杂志2016，Vol.26Issue(18)：6-10,5.DOI:10.3969/j.issn.1005-8982.2016.18.002
骨癌痛大鼠脊髓背根神经节主要组织相容复合物Ⅱ表达变化的研究
Expression of major histocompatibility classIIin spinal dorsal root ganglions of rats with cancerous bone pain
李明 1耿卫国 2刘文明3
作者信息
- 1. 山东省东营市人民医院 护理部，山东 东营 257091
- 2. 山东省东营市立儿童医院，山东 东营 257091
- 3. 滨州医学院附属医院 烧伤整形外科，山东 滨州 256603
- 折叠
摘要
Abstract
Objective To investigate the changes in the expression level of major histocompatibility class II(MHCII) in spinal dorsal root ganglions of rats with cancerous bone pain (CBP). Methods Thirty-three adult virgin female Sprague-Dawley rats weighing 200-220 g, were randomly divided into three groups:naive group ( = 9), sham group ( = 12), and CBP group ( = 12). Walker 256 rat mammary carcinoma cells were inoculated into the right tibial medullary canal to establish a CBP model, whereas the same volume of D-Hanks solution was injected in the sham group. Mechanical paw withdrawal threshold (MPWT) was examined 1 day before and 3, 7, 10 and 14 days after surgery using calibrated von-Frey filaments applied to the center of ipsilateral hind paw. The L3-L5 ipsilateral dorsal root ganglions (DRGs) were collected 14 days after surgery to test the expression level of MHC II by Western blot and immunofluorescent staining. Results The MPWT was not significantly different among the three groups before operation. In the CBP group the MPWT significantly decreased on the 7th day after surgery ( < 0.05), and was significantly lower than that of the naive group ( < 0.05). The immunofluorescent staining identified that MHCII was dominantly expressed by satellite cells in the DRGs of the CBP rats. Furthermore, Western blot results showed that expression level of MHC II was significantly increased in the CBP group, but not in the sham group, as compared with the naive group. Conclusions The changes in the expression level of MHC II in satellite cells of DRGs may be involved in the development of peripheral sensitivity under CBP.关键词
骨癌痛/背根神经节/主要组织相容复合物Ⅱ/外周敏化Key words
cancerous bone pain/dorsal root ganglion/major histocompatibility class II/peripheral sensitivity分类
医药卫生引用本文复制引用
李明,耿卫国,刘文明..骨癌痛大鼠脊髓背根神经节主要组织相容复合物Ⅱ表达变化的研究[J].中国现代医学杂志,2016,26(18):6-10,5.
