中国现代医学杂志2016，Vol.26Issue(18)：11-17,7.DOI:10.3969/j.issn.1005-8982.2016.18.003
色胺酮抑制胸腺基质淋巴细胞生成素促肥大细胞增殖的药理机制*
Pharmacological mechanism of tryptanthrin inhibition of mast cell proliferation promoted by thymic stromal lymphopoietin
摘要
Abstract
Objective To investigate the pharmacological mechanism of tryptanthrin (TR) in suppressing mast cell proliferation promoted by thymic stromal lymphopoietin (TSLP), so as to provide academic evidence for mast cell induced allergic dermatitis (AD) therapy through TR. Methods After HMC-1 cells were stimulated by TSLP, cell proliferation was observed with another treatment of TR, and the relative protein levels were detected as well. The cells were divided into four groups: blank control, TSLP stimulating, TR treating and TSLP combined with TR groups. Cell viability was tested by MTT assay with various concentrations of TR. Cell proliferation effect was verified through BrdU assay and Ki67 mRNA level detection. MDM2, p53, cleaved caspase-3 and cleaved poly-ADP-ribose polymerase (PARP) protein levels were measured by Western blot. IL-7Ra and TSLPR mRNA levels were detected by real-time fluorescent quantitative PCR. Results MTT assay showed TR had no cytotoxicity.BrdU and Ki67 assay indicated that TR evidently suppressed mast cell proliferation promoted by TSLP. After TR treatment, MDM2 protein level decreased in HMC-1 cells and the difference had statistical significance; on the contrary, p53, cleaved caspase and cleaved PARP protein levels significantly elevated. The upstream regulatory protein IL- 7Ra and TSLPR mRNA levels were inhibited obviously with TR treatment. Conclusions TR could suppress mast cell proliferation promoted by TSLP, and exhibits certain potential in AD therapy.关键词
色胺酮/胸腺基质淋巴细胞生成素/人源肥大细胞系- 1细胞/半胱天冬酶- 3/多聚ADP- 核糖聚合酶Key words
tryptanthrin/thymic stromal lymphopoietin/human mast cell line-1 cell/caspase-3/cleavage of poly-ADP-ribose polymerase分类
医药卫生引用本文复制引用
王福银,杜倩,孔丽娟,程春红,王磊..色胺酮抑制胸腺基质淋巴细胞生成素促肥大细胞增殖的药理机制*[J].中国现代医学杂志,2016,26(18):11-17,7.基金项目
国家自然青年科学基金 （）
