中国现代医学杂志2016，Vol.26Issue(18)：22-29,8.DOI:10.3969/j.issn.1005-8982.2016.18.005
MicroRNA-219-5p靶向E-钙黏蛋白调控上皮间质转化抑制肝癌细胞侵袭转移
MicroRNA-219-5p regulates EMT and inhibits invasion and metastasis of hepatoma cells by targeting E-cadherin
朱理辉 1罗勇 2廖文秋 1张琍 1李国庆1
作者信息
- 1. 南华大学附属第二医院 消化内科，湖南 衡阳 421001
- 2. 南华大学附属第二医院 重症医学科，湖南 衡阳 421001
- 折叠
摘要
Abstract
Objective To investigate the molecular mechanism of microRNA-219-5p (miR-219-5p) targeting E-cadherin (CDH1) in the regulation of epithelial mesenchymal trasition (EMT), thus inhibiting the invasion and metastasis of hepatoma cells. Methods Bioinformatics methods were used to determine miRNAs with the best specificity and stability of binding to E-cadherin. The correlation between E-cadherin expression detected by Western blot, and miR-219-5p level by qRT-PCR in 30 hepatoma tissues and 20 normal tissues, respectively, was analyzed. miR-219-5p level was detected by qRT-PCR. E-cadherin and N-cadherin levels were detected by Western blot in high and low metastatic hepatoma cell lines. miR-219-5p mimic, inhibitor and negative control were transfected into HepG2 cell line by Lipofectamine 2000, miR-219-5p expression was detected by qRT-PCR and the expressions of E-cadherin and N-cadherin were detected by Western blot. And the effects of miR-219-5p expression change on invasive and metastatic abilities were also tested by the transwell method. Results Bioinformatics methods showed that miR-219-5p was the best target miRNA with the highest specificity and stability for binding to E-cadherin. miR-219-5p had low expression and E-cadherin had high expression in the HCC, while miR-219-5p had high expression and E-cadherin had low expression in the paracancerous tissues. There were high expression of miR-219-5p, low expression of E-cadherin and high expression of N-cadherin in highly metastatic cell line MHCC97-H. There were low expression of miR-219-5p, high expression of E-cadherin and low expression of N-cadherin in low metastatic cell line MHCC97- L ( < 0.05). Increased miR-219- 5p caused E- cadherin down-regulation and N-cadherin up-regulation. miR-219-5p down-regulation caused E-cadherin up-regulation and N-cadherin down-regulation in HepG2-miR-219-5p-inhibitor cell line ( < 0.05). The invasion cell count was (24±3)/HP in the HepG2-miR-219-5p mimic cells which was significantly lower than that in the control group ( < 0.05). Conclusions miRNA-219-5p can specifically bind to E-Cadherin and regulate the EMT signaling pathway, thus suppress the invasion and metastasis of hepatoma cells.关键词
miRNA/肝癌/侵袭转移/E- Cadherin/上皮间质转化Key words
miRNA/hepatoma/invasion/metastasis/E-cadherin/EMT分类
医药卫生引用本文复制引用
朱理辉,罗勇,廖文秋,张琍,李国庆..MicroRNA-219-5p靶向E-钙黏蛋白调控上皮间质转化抑制肝癌细胞侵袭转移[J].中国现代医学杂志,2016,26(18):22-29,8.
