中国药房2016，Vol.27Issue(27)：3832-3835,4.DOI:10.6039/j.issn.1001-0408.2016.27.29
HPLC法同时测定丹知青娥片中4种成分的含量
Simultaneous Determination of 4 Components in Danzhi Qing’e Tablet by HPLC
摘要
Abstract
OBJECTIVE:To establish a method for the contents determination of psoralen,isopsoralen,psoralenoside and isop-soralenoside in Danzhi qing’e tablet. METHODS:HPLC performed on the column of Eclipse XDB-C18 with mobile phase of metha-nol-water(51∶49,V/V)(isocratic elution,for psoralen and isopsoralen)and acetonitrile-0.1% formic acid(12∶88,V/V)(isocratic elution,for psoralenoside and isopsoralenoside)at a flow rate of 1.0 ml/min,the detection wavelength was 246 nm,column tem-perature was 30℃,and injection volume was 10 μl. RESULTS:The linear range was 3.138-200.8 μg/ml for psoralen(r=0.999 9), 3.175-203.2μg/ml for isopsoralen(r=0.999 9),3.181-101.8μg/ml for psoralenoside(r=0.999 9)and 3.169-101.4μg/ml for isopso-ralenoside (r=0.999 9);RSDs of precision,stability and reproducibility tests were lower than 2%;limits of quantitation were 0.627 5 ng,0.635 0 ng,3.181 0 ng and 3.169 0 ng,the limits of detection were 0.251 0 ng,0.254 0 ng,1.273 0 ng and 1.268 0 ng;recoveries were 95.68%-102.80%(RSD=2.4%,n=6),95.91%-102.10%(RSD=2.3%,n=6),98.64%-99.13%(RSD=0.23%,n=6) and 100.20%-101.70%(RSD=0.69%,n=6),respectively. CONCLUSIONS:The method is simple and accurate, and can be used for the simultaneous determination of psoralen,isopsoralen,psoralenoside and isopsoralenoside in Danzhi qing’e tablet.关键词
丹知青娥片/含量测定/补骨脂素/异补骨脂素/补骨脂苷/异补骨脂苷/高效液相色谱法Key words
Danzhi qing’e tablet/Content determination/Psoralen/Isopsoralen/Psoralenoside/Isopsoralenoside/HPLC分类
医药卫生引用本文复制引用
杨帆,于卉娟,王亚静,杨君君,张波泳,王跃飞,柴欣..HPLC法同时测定丹知青娥片中4种成分的含量[J].中国药房,2016,27(27):3832-3835,4.基金项目
国家自然科学基金资助项目（No.81403060）；“重大新药创制”科技重大专项项目 （）
