中国药房2016，Vol.27Issue(27)：3875-3876,3877,3.DOI:10.6039/j.issn.1001-0408.2016.27.44
快速溶剂萃取-HPLC法测定骨碎补中柚皮苷的含量
Determination of Naringin in Davallia mariesii by Accelerated Solvent Extration-HPLC
黄北雄 1戴柏桉1
作者信息
- 1. 广西梧州食品药品检验所，广西梧州 543002
- 折叠
摘要
Abstract
OBJECTIVE:To develop a new way for the extration and determination of naringin in Davallia mariesii. METH-ODS:Naringin in D. mariesii was extracted by ASE350 accelerated solvent extraction system;HPLC was performed for the con-tent determination of naringin in D. mariesii,the column was Kinetex XB-C18 with mobile phase of methanol-5%acetic acid(25∶75, V/V)at a flow rate of 0.8 ml/min,detection wavelength was 283 nm,column temperature was 40℃,and injection volume was 10 µl. RESULTS:The linear range of naringin was 0.073 0-0.730 0 µg(r=0.999 9);the limit of quantitation was 0.73 μg,the limit of detection was 0.022 μ g;RSDs of precision, stability and reproducibility tests were lower than 2% ;recovery was 98.92%-100.85%(RSD=0.72%,n=6). CONCLUSIONS:The method is simple,accurate and specific,and can be used for the content determination of naringin in D. Mariesii.关键词
高效液相色谱法/骨碎补/柚皮苷/快速溶剂萃取Key words
HPLC/Davallia mariesii/Naringin/Accelerated solvent extraction分类
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黄北雄,戴柏桉..快速溶剂萃取-HPLC法测定骨碎补中柚皮苷的含量[J].中国药房,2016,27(27):3875-3876,3877,3.
