中国药物经济学2016，Vol.11Issue(9)：24-26,3.DOI:10.12010/j.issn.1673-5846.2016.09.004
紫外分光光度法与反相高效液相色谱法测定阿昔洛韦眼用凝胶中阿昔洛韦的含量
Determination of A Silowe in A Silowe Ophthalmic Gel by UV Spectrophotometry and Reversed Phase high Performance Liquid Chromatography
李桂华1
作者信息
- 1. 新疆玛纳斯佳园医院，新疆昌吉回族自治州 832200
- 折叠
摘要
Abstract
Objective To explore the feasibility of the determination of A Silowe in A Silowe ophthalmic gel by UV spectrophotometry and reversed phase high performance liquid chromatography.Methods By UV spectrophotometry, reversed phase high performance liquid chromatography according to the same method of preparing the aciclovir eye gel sample at 252 nm wavelength determination the content of aciclovir.Results Cntent of acyclovir in acyclovir ophthalmic gel(100.49±0.14)% lower than the measured UV spectrophotometry (101.55±0.47)%,the difference was statisticaly significant(P<0.05).Conclusion HPLC method is efective because of the separation-related substances, reducing interference of impurities,the method is simple,accurate and sensitive than UV spectrophotometry,it’is more conducive to the determination content of acyclovir in acyclovir gel.关键词
反相高效液相色谱法/阿昔洛韦/眼用凝胶/紫外分光光度法Key words
HPLC method/Acyclovir/Ophthalmic gel/UV spectrophotometry分类
医药卫生引用本文复制引用
李桂华..紫外分光光度法与反相高效液相色谱法测定阿昔洛韦眼用凝胶中阿昔洛韦的含量[J].中国药物经济学,2016,11(9):24-26,3.
