作物学报2016，Vol.42Issue(9)：1261-1272,12.DOI:10.3724/SP.J.1006.2016.01261
一个新的水稻D1基因等位突变体的遗传鉴定与基因功能分析
Genetic Identification of a NewD1-allelic Mutant and Analysis of Its Gene Function in Rice
摘要
Abstract
Plant height is one of important traits for rice yield. One genetically stable rice mutant,LTH-m3, was isolated from the cv. Lijiangxintuanheigu (LTH)-derived mutant population by mutagenesis using ethylmethane sulfonate (EMS).LTH-m3 was involved in the pathways of gibberellic acid (GA) and brassinosteroid (BR), and showed no sensitiveness to exogenous GA (GA3) and significantly reduced sensitiveness to exogenous BR (eBL) compared with the wild type. The genetic analysis, gene cloning and transgenic complementary test confirmed thatLTH-m3 was a newd1-allelic mutant with small grain and dwarf phenotypes, and a single base was mutated (G2522→A2522) in the functional dwarf geneD1 at the conjunction site of its sixth exon and intron, which caused excision of the sixth exon in mRNA and premature termination of theD1encoded Gα protein, resulting in mutated phenotypes in the mutant. The further study showed that theD1 mutation caused obvious expression change of some dwarf genes such asSD1 andSLR1 in the mutant, and could affect the GA and BR pathways in their feedback regulations and signaling trans-ductions in plant cells. The mutant overcome the defects of the universally blast-susceptible cv. LTH, such as too tall plant, soft stem and easy lodging, and could be utilized as an improved substitute of LTH in the future rice blast researches. The mutatedD1 gene identified from theLTH-m3mutant may be useful for further study of Gα functions and signaling pathways of GA and BR.关键词
水稻/突变体/矮秆基因/基因克隆/基因功能Key words
Rice/Mutant/Dwarf gene/Gene cloning/Gene function引用本文复制引用
王翠红,张欣,郭秀平,雷财林,马建,王帅,田鹏,岂长燕,赵志超,王久林,王洁,程治军..一个新的水稻D1基因等位突变体的遗传鉴定与基因功能分析[J].作物学报,2016,42(9):1261-1272,12.基金项目
本研究由国家公益性行业(农业)科研专项经费(201203014)和中国农业科学院科技创新团队项目“作物功能基因组”资助。This study was supported by the Special Fund for Agro-scientific Research in the Public Interest Program of China (201203014) and the Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences “Crop Functional Genomics” （农业）
