作物学报2016，Vol.42Issue(9)：1273-1281,9.DOI:10.3724/SP.J.1006.2016.01273
野生小豆和栽培小豆microRNA全基因组鉴定与比较分析
Global Identification and Comparison of MicroRNA in Wild and Cultivated Adzuki Bean
摘要
Abstract
MicroRNAs (miRNAs) are a class of small regulatory RNAs with the function as crucial regulators that repress the expression of their target genes at the transcriptional or post-transcriptional level. Global identification of miRNA is important for the functional study. Adzuki bean (Vigna angularis) genome sequencing has been finished recently, but lack of a systematical analysis of miRNA. In this study, we performed deep miRNA sequencing in wild (CW108) and cultivated (JN6) adzuki beans. Our investigation not only identified the miRNAs that shared by these two species, but also found miRNAs that specifically existed in individual accession. Through comparison of the miRNA expression and their target gene predictions between the wild and cultivated adzuki beans, we found that the miRNA was diverged between them, which may be associated with plant-pathogen interaction pathway. Our study provided a valuable resource for functional analysis in adzuki bean.关键词
miRNA/栽培小豆(Vigna angularis)/野生小豆(Vigna angularis var.nipponensis)Key words
miRNA/Wild adzuki bean (Vigna angularis)/Cultivated adzuki bean (Vigna angularis var.nipponensis)引用本文复制引用
马燕明,陈春海,杨凯,李奕松,赵波,李江,李永强,万平..野生小豆和栽培小豆microRNA全基因组鉴定与比较分析[J].作物学报,2016,42(9):1273-1281,9.基金项目
本研究由国家自然科学基金项目(31371694)资助。This study was supported by National Natural Science Foundation of China (31371694) （31371694）
