作物学报2016，Vol.42Issue(9)：1298-1308,11.DOI:10.3724/SP.J.1006.2016.01298
2个玉米ZmCRY1a基因的克隆及其响应光质处理的表达模式
Molecular Cloning of Two Maize (Zea mays)CRY1a Genes and Their Expres-sion Patterns of in Response to Different Light Treatments
摘要
Abstract
Cryptochromes are blue light receptors that regulate the development of growth and circadian clock in plants. To stress the functions of crytochrome 1 (CRY1) on photomorphogenesis and flowering regulation in maize (Zea mays L.), we isolated the cDNA clones of twoZmCRY1a genes from inbred line B73 by homologous cloning, and designated asZmCRY1a1 and ZmCRY1a2. The length of bothZmCRY1a coding DNA sequences were 2124 nucleotides, which encoded 707 amino acid residues. Bioinformatics analyses were employed to predict their function domains and to build a phylogenetic relationship tree among plant CRY1 homologs by the DNAMAN software and the NCBI blast. The two ZmCRY1a proteins possessed three function do-mains: DNA photolyase, FAD binding, and crytochrome C domains. Phylogenetic analysis indicated that the two ZmCRY1a pro-teins belonged to the same branch with OsCRY1a, while showing low similarity to other CRY1 proteins from dicotyledonous species, such asA. thaliana andGlycine max. The transcription abundances of two ZmCRY1a genes in different organs and in response to light treatments were detected using quantitative RT-PCR (qRT-PCR). qRT-PCR assays indicated that the two ZmCRY1a genes were highly expressed in leaf with 52.1 or 6.2 times higher thanZmCRY1a1 abundance in root, respectively. The transcription abundances of the both genes were very high under different continuous light conditions, especially in blue and far-red light. Although encoding blue light receptors, they both greatly responded to dark-to-far-red and dark-to-red transitions. In addition, their transcription abundances could also respond to photoperiod treatment (both long-day and short-day conditions). In long-day condition, The transcription abundances ofZmCRY1a1andZmCRY1a2 hadfive four peaks, respectively. In short-day condition, bothZmCRY1a genes had two big peaks which happened at 10 h and 14 h after transition into darkness. Our results suggest that bothZmCRY1a genes may be involved in seedling de-etiolation and flowering time control, thus their roles in crop improvement are worthy of more exploration in the future.关键词
玉米/隐花色素/光信号转导/基因克隆/表达模式Key words
Zea mays.L/Cryptochrome/Light signaling transduction/Gene cloning/Expression pattern引用本文复制引用
闫蕾,杨建平,杨宗举,苏亮,肖阳,郭林,宋梅芳,孙蕾,孟凡华,白建荣..2个玉米ZmCRY1a基因的克隆及其响应光质处理的表达模式[J].作物学报,2016,42(9):1298-1308,11.基金项目
本研究由国家重点研发计划试点专项项目(SQ2016ZY03002918),国家转基因生物新品种培育重大专项(2016ZX08010002-003-002),国家自然科学基金项目(3157026),北京市自然科学基金(重点)项目(6151002)和中国农业科学院科技创新工程项目资助。This study was supported by the Special Project of National Key Research and Development Project (SQ2016ZY03002918), the Genetically Modified Organisms Breeding Major Projects of China (2016ZX08010002-003-002), the National Natural Science Foundation of China (31570268), the Beijing Natural Science Foundation (6151002), and the Agricultural Science and Technology Innovation Program (ASTIP) （SQ2016ZY03002918）
