作物学报2016，Vol.42Issue(9)：1309-1318,10.DOI:10.3724/SP.J.1006.2016.01309
大豆籽粒大小与形状性状的QTL定位
Mapping Quantitative Trait Loci for Seed Size and Shape Traits in Soybean
摘要
Abstract
Seed size and shape not only relate to seed yield and quality, but also affect mechanical seeding in soybean (Glycine maxL.). In this study, F6:8 and F6:9populations derived from Jidou 12 × Heidou were used to analyze the genetic character and detect quantitative trait loci (QTLs) for seed length, seed width, seed thickness, seed length-to-width ratio, seed length-to-thickness ratio, and seed width-to-thickness ratio. Softwares WinQTLCart 2.5, QTLNetwork 2.1 and IciMapping 4.1 were used to identify the additive, epistatic and environmentally interacted QTLs for seed size and shape related traits. As results, the heritability of the six traits varied from 64.01% to 79.57%. A total of 38 additive QTLs were identified to be located on 12 chromosomes, with the heritability varying from 2.21% to 10.71%.Eight of them(qSL-17-1, qSL-18-1, qSW-6-1, qST-2-1, qST-6-1, qSLT-2-2, qSWT-2-1,andqSWT-20-1)were identified using three methods, simultaneously. In the meantime, seven pairs of additive × additive epistasis were detected and the heritability of epistasis pairs ranged from 0.78% to 6.20%. Additionally, the effects of QTL by environment interaction ranged from 0.0005% to 0.3900%. The QTLs identified using different mapping soft-wares in this study could provide a reliable theoretical basis for marker-assisted selection breeding.关键词
大豆/粒形/QTL/上位性互作Key words
Soybean/Seed shape/Quantitative trait locus/Epistasis引用本文复制引用
陈强,闫龙,邓莹莹,肖二宁,刘兵强,杨春燕,张孟臣..大豆籽粒大小与形状性状的QTL定位[J].作物学报,2016,42(9):1309-1318,10.基金项目
本研究由国家自然科学基金项目(31471522),河北省自然科学基金项目(C2015301012),国家高技术研究发展计划(863计划)项目(2012AA101106),国家现代农业产业技术体系建设专项(CARS-004-PS06)和国家转基因生物新品种培育重大专项(2014ZX0800402B)资助。This study was supported by the National Natural Science Foundation of China (31471522), the Natural Science Foundation of Heibei Prov-ince (C2015301012), the National High-tech Research and Development Program of China (2012AA101106), the China Agriculture Research System (CARS-004-PS06), and the National Major Project for Developing New GM Crops (2014ZX0800402B) （31471522）
