作物学报2016，Vol.42Issue(9)：1342-1351,10.DOI:10.3724/SP.J.1006.2016.01342
海岛棉转录因子基因GbMYB60的克隆、表达及其抗逆性分析
Cloning, Expression, and Functional Analysis of Transcription Factor Gene GbMYB60in Cotton
摘要
Abstract
MYB transcription factors are conserved proteins in all eukaryotic cell, which play important roles in plant growth, development, metabolism, abiotic and biotic stress resistance, as well as phytohormone-mediated signal transduction. In this re-search, a MYB gene was isolated from the sea-island cotton cultivar Hai 7124. This gene was namedGbMYB60 based on the se-quence similarity search and phylogenetic analysis. The full-length ofGbMYB60 coding sequence is 990 bp, andGbMYB60 en-codes a 36.9 kD R2R3-type MYB protein, which is specifically located in nucleus of plant cell.GbMYB60 was preferentially ex-pressed in leaf and induced by abiotic stresses (such as salt, mannitol, cold, and heat) and phytohormones (abscisic acid, ethephon, methyl jasmonate, and salicylic acid) treatments, but the general expression ofGbMYB60 was low in all tissues. Salt and mannitol tolerances were analyzed in control andGbMYB60silenced-cotton generated by virus-induced gene silencing system, and the results showed thatGbMYB60 positively regulated cotton tolerance to salt but not to mannitol.关键词
棉花/MYB转录因子/盐/甘露醇/非生物胁迫Key words
Cotton/MYB transcription factor/Salt/Mannitol/Abiotic stress引用本文复制引用
高巍,刘会利,田新权,张慧,宋洁,杨勇,龙璐,宋纯鹏..海岛棉转录因子基因GbMYB60的克隆、表达及其抗逆性分析[J].作物学报,2016,42(9):1342-1351,10.基金项目
本研究由国家重点基础研究发展计划项目(2012CB1143001),棉花生物学国家重点实验室开放课题(CB2015A31)和河南省教育厅项目(15A180028,15A180029)资助。This study was supported by the National Basic Research Program of China (2012CB1143001), State Key Laboratory of Cotton Biology Open Fund (CB2015A31), and Henan Provincial Educational Department Foundation of China (15A180028,15A180029) （2012CB1143001）
