作物学报2016，Vol.42Issue(9)：1374-1380,7.DOI:10.3724/SP.J.1006.2016.01374
高温胁迫对Bt棉蕾中杀虫蛋白含量及氮代谢的影响
Effect of High Temperature Stress on Bt Insecticidal Protein Content and Nitrogen Metabolism of Square in Bt Cotton
摘要
Abstract
To explore the reason for low insect resistance under high temperature climate in Bt cotton production, we selected two Bt cotton cultivars as experimental material with treatments of 38℃ for three, five and seven days in 2011, and 38℃/25℃ at day /night for four, seven and ten days, respectively at the artificial climate chamber in 2012. The results showed that the Bt insecti-cidal protein contents declined within seven days under 38℃, with the largest reduction occurred within three days. Compared with control, the square Bt insecticidal protein contents decreased by 18.71% and 26.54% for cultivar Sikang 3 (SK-3) and Sikang 1 (SK-1), respectively. Under 38℃/25℃ at day/night, the square Bt insecticidal protein contents had no obvious reduction within four days, and significant reduced after seven days with the reduction of 11.32% for SK-3 and 14.18% for SK-1. Under the treat-ment conditions the soluble protein contents, glutamate pyruvate transaminase (GPT) activities reduced, but the free amino acid contents, protease activities increased. There existed significant negative correlation of insecticidal protein content with free amino acid contents, and protease activities; and significantly positive correlation of insecticidal protein content with soluble pro-tein contents, and GPT activities under high temperature stresses. Therefore, the reduced synthesis and the enhanced degradation for protein in the square under the high temperature condition resulted in the decrease of soluble protein content, including Bt insecticidal protein content. The larger reductions of the square Bt insecticidal protein content and nitrogen metabolic strength were detected in cultivar SK-1 as confound with cultivar SK-3 under the high temperature treatments.关键词
Bt棉/蕾/Bt杀虫蛋白/氮代谢/高温胁迫Key words
Bt cotton/Square/Bt insecticidal protein/Nitrogen metabolism/High temperature stress引用本文复制引用
衡丽,李亚兵,胡大鹏,王桂霞,吕春花,张祥,陈源,陈德华..高温胁迫对Bt棉蕾中杀虫蛋白含量及氮代谢的影响[J].作物学报,2016,42(9):1374-1380,7.基金项目
本研究由国家自然科学基金项目(31171479,31301263,31471435),教育部博士学科点专项基金(博导类)项目(20113250110001),江苏省三新工程项目(SXGC(2014)317)和江苏省高等教育优势学科发展计划项目资助。The study was supported by the National Natural Science Foundation of China (31171479,31301263,31471435), the Ministry of Education Research Fund for the Doctoral Program (20113250110001), Jiangsu “Three-innovation” Agricultural Project [SXGC(2014)317], and the Development Plan of Higher Education Advantaged Discipline in Jiangsu Province （31171479,31301263,31471435）
