作物学报2016，Vol.42Issue(9)：1391-1401,11.DOI:10.3724/SP.J.1006.2016.01391
外源多胺对小麦小花退化的调控机制
Effect of Exogenous Polyamines on Mechanism of Floret Degeneration in Wheat
摘要
Abstract
Kernel number per spike has a close relationship with floret degeneration in wheat, which is regulated greatly by polyamines (PAs), one of important plant growth regulators. In this study, we applied exogenous spermine (Spm), spermidine (Spd) and putrescine (Put) to the young spikes of cultivars Shuangda 1 (large-spike type) and Xinong 538 (small-spike type) to investigate the effects of PAs on floret degeneration, endogenous hormones, and the carbon and nitrogen changes in wheat floret. The results indicated that exogenous Spd and Spm had similar effects on floret degeneration whereas exogenous Put was in func-tion. In the treatments with external Spd and Spm applied, the floret degeneration was inhibited and the number of fertile florets increased significantly. In the external Put treatment, floret degeneration aggravated and the fertile floret number decreased sig-nificantly. Such influence by external PAs was different in upper and lower florets of a spike and the upper florets (inferior florets) showed greater effects than the lower ones (superior florets). The concerntrations of endogenous Spd and Spm, zeatin (Z) + zeatin ridoside (ZR), and the ratio of Z+ZR-to-abscisic acid (ABA)in inferior florets increased after applying exogenous Spd and Spm. However, the evolution rate of endogenous ethylene (ETH) in inferior floret decreased. As a result, the total soluble sugar and protein contents increased significantly in inferior floret. In contrast, exogenous Put showed reverse effects compared to Spd and Spm. Our results indicate that PAs were involved in the regulation of floret degeneration by changing the endogenous hormone concerntrations and the carbon and nitrogen metabolism in wheat plants.关键词
多胺/小麦/小花退化/激素/可溶性总糖/可溶性蛋白Key words
Polyamine/Wheat/Floret degeneration/Hormone/Total soluble sugar/Soluble protein引用本文复制引用
吕晓康,温晓霞,廖允成,刘杨..外源多胺对小麦小花退化的调控机制[J].作物学报,2016,42(9):1391-1401,11.基金项目
本研究由国家自然科学基金项目(31301260),国家科技支撑计划项目(2015BAD22B03-05)和中国博士后科学基金面上项目(2015M572604)资助。This study was supported by the National Natural Science Foundation of China (31301260), the National Key Technology R&T Program of China (2015BAD22B03-05), and the China Postdoctoral Science Foundation (2015M572604) （31301260）
