作物学报2016，Vol.42Issue(9)：1411-1416,6.DOI:10.3724/SP.J.1006.2016.01411
101份中国甘蔗主要育种亲本褐锈病抗性鉴定及Bru1基因的分子检测
Identification of Resistance to Brown Rust and Molecular Detection ofBru1 Gene in 101 Main Sugarcane Breeding Parents in China
摘要
Abstract
Sugarcane brown rust induced byPuccinia melanocephala is an important global disease.Bru1is a major brown rust resistance gene with a broad-spectrum resistance against various brown rust pathogens originating from different countries. In order to assess the resistance level of main sugarcane breeding parents toPuccinia melanocephala, and determine the distribution of theBru1 gene in these breeding parents in China, 101 main sugarcane breeding parents collected from the Chinese National Nursery of Sugarcane Germplasm Resources were evaluated and identified for their brown rust resistance at the seedling stage and for the presence of the brown rust resistance geneBru1.Bru1 was detected to present in 48 (47.5%) resistant materials out of the 101 tested parental lines, indicating that brown rust resistance in main sugarcane breeding parents in China is primarily controlled byBru1. The absence ofBru1in the other 29 resistant and 24 susceptible parental lines suggests that they may carry brown rust resistance-associated genes other thanBru1. These results may facilitate future efforts to breed varieties with improved resistance to brown rust and may provide resistant parental lines for selecting and planting elite resistant varieties to effectively control brown rust disease in sugarcane.关键词
甘蔗/主要育种亲本/抗褐锈病基因Bru1/分子检测Key words
Sugarcane/Main breeding parents/Brown rust resistance geneBru1/Molecular detection引用本文复制引用
李文凤,王晓燕,黄应昆,张荣跃,单红丽,罗志明,尹炯..101份中国甘蔗主要育种亲本褐锈病抗性鉴定及Bru1基因的分子检测[J].作物学报,2016,42(9):1411-1416,6.基金项目
本研究由国家现代农业产业技术体系建设专项(CARS-20-2-2)和云南省现代农业产业技术体系建设专项资助。This study was supported by China Agriculture Research System (CARS-20-2-2) and the Agriculture Research System of Yunnan Province （CARS-20-2-2）
