安徽农业科学2016，Vol.44Issue(23)：90-92,95,4.
Vc对猪体细胞克隆胚胎体外及体内发育的影响
Effects of Vitamin C on the Development of Porcine Somatic Cloned Embryos in vitro and in vivo
摘要
Abstract
Objective] To improve the ability to generate cloned pigs and to optimize the culture system in vitro and in vivo.[Method] The por-cine SCNT embryos were cultured in the medium with different vitamin C concentrations .Then the blastocyst formation rate and cell number per blastocyst were compared.Proper concentration was screened,which was used to treat cloned embryos.After surgery transfer,farrowing rate and litter size were calculated.[Result] After stimulation,cloned embryos were treated by 40μg/mL Vc for 22 h;culture in vitro did not significantly enhance the blastocyst rate,but the blastocyst cell number was significantly increased.Results of in vivo test showed that adding Vc did not signifi-cantly enhance the pregnancy rate,but increased the average litter size.There were significant differences in the cloning efficiency.[Conclusion] Vc treatment is beneficial to the development of porcine SCNT embryos both in vivo and in vitro.关键词
Vc/猪/克隆胚胎/发育Key words
Vitamin C/Porcine/Cloned embryos/Development分类
农业科技引用本文复制引用
周荣,吴珍芳,罗绿花,麦然标,余婉娴,贺晓燕,纪红美,石俊松,王青来,蔡更元..Vc对猪体细胞克隆胚胎体外及体内发育的影响[J].安徽农业科学,2016,44(23):90-92,95,4.基金项目
国家转基因生物新品种培育项目（2016ZX08006002；2016ZX08006003）。 （）
