安徽农业科学2016，Vol.44Issue(23)：99-102,4.
SDS－PAGE电泳在鉴别用牛乳掺伪的新疆特种乳中的应用
Use of SDS-PAGE in Detecting Bovine Milk Content in Adulterated Xinjiang Non-bovine Milk
摘要
Abstract
Objective] The aim was to use SDS-PAGE to detect adulterated non-bovine milk and measure bovine milk content and confirm the lowest adulteration ratio.[Method]By using different ratio of(1∶1, 1∶4, 1∶10, 1∶20, 1∶100, 1∶200 and 1∶400(Vbovine milk/Vnon-bo-vine milk) )adulterated non-bovine milk, whey protein was isolated and SDS-PAGE was conducted to locate the indicating protein then use gray analysis to detect adulteration and calculate bovine milk content.[Result] The SDS-PAGE can efficiently separate each animal’s whey protein as well as bovine whey protein from adulterated whey.[Conclusion] The lowest adulterating ratio can be detected with SDS-PAGE as 1∶10 (Vbovine milk/Vnon-bovine milk).关键词
SDS-PAGE电泳/特种乳/灰度分析Key words
SDS-PAGE/Non-bovine milk/Gray analysis分类
农业科技引用本文复制引用
阿力木·吾布力,杨洁,苏力坦·阿巴百克力..SDS－PAGE电泳在鉴别用牛乳掺伪的新疆特种乳中的应用[J].安徽农业科学,2016,44(23):99-102,4.基金项目
国家自然科学基金项目“基于特种乳差异蛋白组分析建立乳间掺伪检测方法研究”（31471645）。 （）
