安徽农业科学2016，Vol.44Issue(23)：119-121,3.
苯酚硫酸法与蒽酮硫酸法测定托盘根多糖含量的比较
Comparison of Phenol-sulfuric Acid and Anthrone-sulfuric Acid Methods for Determination of Polysaccharide in Rubus crataegiflolius Bunge
摘要
Abstract
Objective] The phenol-sulfuric acid method and anthrone-sulfuric acid method were compared on determination of the polysac-charide content in Rubus crataegiflolius Bunge..[Method] The conversion factor was measured with refined polysaccharide for glucose, and comprehensive evaluation was carried out by linear relationship, precision, reproducibility, stability and recovery rate.[ Result] The results showed that the linear relationship between glucose concentration and absorbance was good in the range of 5-40 μg/mL, and the average recov-ery rate was equal to or higher than 97% with good reproducibility (RSD=1.7%), the content of polysaccharide determined by phenol-sulfu-ric acid method and anthrone-sulfuric acid method were 3.78% and 4.02% .[ Conclusion] Phenol-sulfuric acid and anthrone-sulfuric acid methods are accurate and reliable, and suitable for determination of polysaccharide in R.crataegiflolius.关键词
托盘根/多糖/苯酚-硫酸法/蒽酮-硫酸法Key words
Rubus crataegiflolius Bunge./Polysaccharides/Phenol-sulfuric acid method/Anthrone-sulfuric acid method分类
农业科技引用本文复制引用
郑旭伟,黄占波,楚冬海,王晓宇,宁雪,黄丽莉..苯酚硫酸法与蒽酮硫酸法测定托盘根多糖含量的比较[J].安徽农业科学,2016,44(23):119-121,3.基金项目
辽宁科技学院博士科研启动基金（1406B05）；辽宁省大学生创新训练项目（201611430024）。 （）
