北华大学学报（自然科学版）2016，Vol.17Issue(5)：611-615,5.DOI:10.11713/j.issn.1009-4822.2016.05.011
白藜芦醇对卵巢癌细胞株SKOV-3细胞周期的影响及诱导其凋亡的机制研究
On Effect of Resveratrol on the Cell Cycle of Ovarian Cancer Cell Strain SKOV-3 and the Apoptosis Mechanism Induced by Resveratrol
摘要
Abstract
Objective To investigate the effects of resveratrol on the cell cycle of human ovarian cancer cell SKOV-3,and to explore the apoptosis mechanism of human ovarian cancer cell SKOV-3 induced by resveratrol. Method The experimental group treated with different concentrations of resveratrol (20,40 and 80 μmol/L) and the cell control group were set. The apoptosis rates of SKOV-3 at different stages in the cell cycles were analyzed by flow cytometry after PI ( propidium iodide) single staining. The apoptosis of human ovarian cancer cell SKOV-3 induced by resveratrol was tested by flow cytometry ( Annexin-v/PI double labeling staining) and analyzed by scatter plot. The effect of resveratrol on the expression of apoptosis-related protein Caspase-3 in SKOV-3 cells was detected by immunohistochemical method. Results After PI ( propidium iodide ) single staining,the flow cytometry results suggested that resveratrol could arrest the cell cycle of ovarian cancer SKOV-3 in S phase. Three scatter plot indicated that resveratrol could induce SKOV-3 early apoptosis,and this effect was positively correlated to the concentration of resveratrol. Resveratrol was able to increase the expression of Caspase-3 in human ovarian cancer SKOV-3 cells. Conclusion Resveratrol can block the cell cycle and induce the early apoptosis of SKOV-3,which may relate to the activation of caspase pathway.关键词
白藜芦醇/SKOV-3细胞/凋亡/流式检测/免疫组织化学Key words
Resveratrol/SKOV-3 cells/apoptosis/flow cytometry( FCM)/immunohistochemistry分类
医药卫生引用本文复制引用
唐锐先,张颖,张巍..白藜芦醇对卵巢癌细胞株SKOV-3细胞周期的影响及诱导其凋亡的机制研究[J].北华大学学报（自然科学版）,2016,17(5):611-615,5.基金项目
吉林省教育厅科学技术研究项目(2014364) （2014364）
