北华大学学报（自然科学版）2016，Vol.17Issue(5)：616-619,4.DOI:10.11713/j.issn.1009-4822.2016.05.012
丹参酮ⅡA通过调控PKC/CyclinD1通路抗大鼠心肌成纤维细胞增殖作用研究
Inhibition of Taishinone ⅡA on Cardiac Fibroblasts Proliferation through PKC/CyclinD1 Pathways in Rats
摘要
Abstract
Objective To observe the effects of TSNⅡA and PKC inhibitor chelerythrine ( Chele ) on the proliferation,cell cycle,and expression of collagen I,PKC and cyclinD1 protein in neonatal cardiac fibroblasts ( CFb) induced by Angiotensin Ⅱ( AngⅡ) and its molecular mechanisms. Method The cultured CFbs of neonatal rats were divided into control group, AngⅡgroup, Chele+AngⅡgroup, Chele+Ang+TSN group, and Ang+TSN group. CFb was isolated by trypsin digestion method and cultured by differential adhesion. MTT colorimetric assay was adopted to evaluate cell proliferation,immunocytochemical( IC) staining method was used to measure collagen I contents, flow cytometry was used to detect the cell cycle, and Western blot ( WB ) was applied to detect the expression of PKC and CyclinD1 . Results Compared with those in AngⅡ group,the CFb proliferation rate was decreased dramatically ( P<0 . 05 or P<0 . 001 ) , the collagenI content was significantly reduced (P<0. 05 or P<0. 01),the G0/G1 ratio was obviously elevated,the percentage of cells in S phase was significantly reduced (P<0. 05 or P<0. 01),and the PKC and cyclinD1 protein expressions were inhibited (P<0. 05 or P<0. 01). TSN and Chele can significantly inhibit the CFb proliferation secretion of cyclinD1 ,and the mechanism can be attributed to inhibiting PKC-CyclinD1 signal pathway.关键词
丹参酮ⅡA/白屈菜红碱/CyclinD1/心肌成纤维细胞Key words
Taishinone( TSN)ⅡA/Chelerythrine( Chele)/cyclinD1/cardiac fibroblast分类
医药卫生引用本文复制引用
付晓杰,孙红霞..丹参酮ⅡA通过调控PKC/CyclinD1通路抗大鼠心肌成纤维细胞增殖作用研究[J].北华大学学报（自然科学版）,2016,17(5):616-619,4.基金项目
吉林省科技发展计划项目(200705355) （200705355）
