北华大学学报（自然科学版）2016，Vol.17Issue(5)：620-623,4.DOI:10.11713/j.issn.1009-4822.2016.05.013
大鼠坐骨神经切断后经皮低频高强度电刺激相应脊髓节段中GAP-43表达的变化
Changes of GAP-43 Expression in the Spinal Cord Segments of Rats after a Low-frequency and High-intensity Electrical Stimulation Following the Sciatic Nerve Transection
摘要
Abstract
Objective To investigate the expression of GAP-43 in the spinal cord of adult Wister rats after a low-frequency and high-intensity electrical stimulation following the sciatic nerve transection. Method 60 Healthy adult male Wister rats were randomly divided into experimental group and control group. The rats in the experimental group were given a low-frequency and high-intensity electrical stimulation following the sciatic nerve transection,and on week 1,2,4,8,16 and 12 after operation,the rats were sacrificed and their L4 ~L6 spinal cord tissues were taken,and then changes of GAP-43 expression in the corresponding segments of spinal cord tissues were detected by immunohistochemistry technique and the data were analyzed by an image analysis system. Results In the control group,the expression of GAP-43 in the anterior horn cells on week 1 was significant,on week 4 reached the peak,on week 5~8 was down-regulated gradually,and on week 9~12 could be still found in a small amount,showing a weakly positive expression. In the experimental group,the expression of GAP-43 in the anterior horn cells on week 1 was significant,and on week 2 reached the peak,and on week 4~16 could be still seen,showing a positive expression. Conclusion The GAP-43 expression of the anterior horn motor neurons in the spinal cord of adult rats can be significantly increased by the sciatic nerve transection. It is proved that the regeneration ability of neurons in the peripheral nerve injury is enhanced, but the time is very short. The expression of GAP-43 was significantly increased in both the duration and the amount after a low-frequency and high-intensity electrical stimulation therapy.关键词
脊髓/外周神经/电刺激/生长相关蛋白-43Key words
spinal cord/peripheral nerve/electrical stimulation/growth-associated protein-43分类
医药卫生引用本文复制引用
朱春雷,何娜,刘丽丽,马跃..大鼠坐骨神经切断后经皮低频高强度电刺激相应脊髓节段中GAP-43表达的变化[J].北华大学学报（自然科学版）,2016,17(5):620-623,4.基金项目
吉林市科技发展计划项目(201339017 （）
201437029 （）
201536070 （）
201536068) （）
吉林省卫生厅科研计划项目(20132030) （20132030）
