动物医学进展2016，Vol.37Issue(9)：5-9,5.
两株不同致病力 H9N2亚型禽流感病毒反向遗传操作系统的构建
Establishment of Reverse Genetics System for Two Different Virulences of H9N2 Subtype Avian Influenza Virus
摘要
Abstract
In order to obtain two stains of rescued H9N2 viruses,which showed different virulences to chick-ens,two sets of eight plasmid system were established.The rescued virus was generated by reserve genetic technique.The full genome of rescued virus was sequenced,and the results showed the rescued virus carried 100% homogeny whth their wild type.The in vitro experiment indicated the rescued virus carried identical replication ability in chicken embryos and MDCK celsl.The animal experiment in vivo showed the virus maintained the same pathogenicity to embryos and SPF chickens.The above experiments proved the reverse genetic operating systems were established,which laid a solid foundation for researching the molecular mechanism of pathogenicity of H9N2 influenza virus.关键词
H9N2亚型禽流感病毒/强致病性/反向遗传/感染性克隆Key words
H9N2 subtype avian influenza virus/higher pathogenicity/reverse genetics/infectious clone分类
农业科技引用本文复制引用
范丹丹,张毅,刘琳琳,王冬冬,王建琳,徐守振,毕玉海,尹燕博..两株不同致病力 H9N2亚型禽流感病毒反向遗传操作系统的构建[J].动物医学进展,2016,37(9):5-9,5.基金项目
国家自然科学基金项目（31272535）；山东省现代农业产业技术体系家禽创新团队建设项目 （）
