动物医学进展2016，Vol.37Issue(9)：16-20,5.
低血清培养 PK-15细胞及其增殖猪圆环病毒2型的研究
Study on PK-15 Cells Cultivated with Low Serum Culture and Culture Proliferation of Porcine Circovirus Type 2
摘要
Abstract
In this study,the low serum media containing 6%,3%,2% and 1% sera,respectively,were used to culture PK-15 cells and to proliferate the porcine circovirus type 2.The results showed that PK-15 cells were in good growth state in concentrations of 6%,3% and 2% sera culture medium,respectively,but when the concentration of the serum was decreased to 1%,the cells were in poor attachment and growth. Meanwhile,the densities of PK-15 cells at 0 h,24 h,48 h and 72 h time point in concentrations of 6%,3%and 2% serum culture medium was not significantly different with the control.Thereby,the culture media containing 2% serum were selected for cell cultivation and virus yield,and the virus titers were determined by indirect immunofluorescent assay.The results showed that the PCV-2 titer of the low serum culture was 106.5 TCID50/mL,a little bit higher than that of the control (10 6.375 TCID50/mL),but the difference was not significant.In this study,the PK-15 cell culture aging system with low serum culture medium for PCV-2 proliferation was established,which laid the foundation for the research of PCV-2.关键词
PK-15细胞/低血清培养基/猪圆环病毒2型/增殖/间接免疫荧光Key words
PK-15 cell/low serum medium/PCV-2/proliferation/indirect immuno fluorescent assay分类
农业科技引用本文复制引用
张瑞永,管宇,吴信明,梅建国,姚春阳,沈志强,雷连成..低血清培养 PK-15细胞及其增殖猪圆环病毒2型的研究[J].动物医学进展,2016,37(9):16-20,5.基金项目
山东省重点研发计划项目 （）
