动物医学进展2016，Vol.37Issue(9)：21-25,5.
BHV-1在牛胚气管细胞中的增殖规律研究
Study on Proliferation Profile of BHV-1 in Bovine Embryonic Tracheal Cells
摘要
Abstract
To reveal the proliferation profile of bovine herpesvirus 1 (BHV-1)in bovine embryonic tracheal (EBTr)cells,the specific primers and probe were designed and TaqMan real-time PCR method was devel-oped in this study.The proliferation of the virus in EBTr cells infected with BHV-1 were detected at 6,12, 24,48,72,96,120,144 h and cytopathic effect (CPE)was observed in the corresponding time points.The results showed that CPE were observed when the EBTr cells were inoculated by 100 TCID50 BHV-1 at 48 h post infection (PI).CPE became clear at 72 h PI,most of the cells became round and began to fall off from flasks at 120 h PI,most of the cells were disintegrated at 144 h PI.The results of real time PCR assay showed that BHV-1 were proliferated slowly when the cells infected within 24 hours,and rapidly during 48 to 96 hours.The load of BHV-1 was still increased,but the growth rate became slowed down during 120 h-144 h.In conclusion,not only BHV-1 can produce CPE in EBTr cells,but also the CPE was consistent with the proliferation profile of viral DNA.The results can provide basic data for further study of the pathogenic mechanism of BHV-1 in EBTr cells.关键词
牛疱疹病毒Ⅰ型/牛胚气管细胞/增殖规律/实时荧光定量PCRKey words
Bovine herpesvirus 1/EBTr/proliferation profile/real-time PCR分类
农业科技引用本文复制引用
徐丽媛,张志飞,周伟光,呼凤丽..BHV-1在牛胚气管细胞中的增殖规律研究[J].动物医学进展,2016,37(9):21-25,5.基金项目
国家自然科学基金项目（30860202，31260597）；内蒙古自治区自然科学基金项目 （）
