动物医学进展2016，Vol.37Issue(9)：31-34,4.
陕西榆林某羊场羊口疮病毒的分离鉴定
Isolation and Identification of Orf Virus in a Goat Farm of Yulin Shaanxi
摘要
Abstract
In order to obtain a wild strain of Orf virus in Yulin,samples of the lip scab from a lamb with clinical symptoms of Orf in a goat farm in Yulin city were collected.After grinded and repeated freezing and thawing,the supernatant was inoculated in calf testis primary cells by passage culture,and then the virus was identified as Orf virus through the observation of cytopathic effects,PCR detection and animal regres-sion test.The results suggested that calf testis primary cells infected with scab by grinding fluid displayed cytopathic effects at the fourth passages,and the virus titer was 107.66 TCID50/mL.B2L gene of isolated vi-rus was cloned and sequenced,and the homologous analysis revealed an identity of 99% with Gansu (KC485343.1)strain and Fujian (KC568399.1)strain.The 2 month-old lambs were inoculated with the cell cultures for the infection experiment,and typical Orf clinic symptoms such as papules and pustules ap-peared in lips and inguen after virus inoculation.In conclusion,a wild strain of Orf virus in Yulin was isola-ted in this study.关键词
羊口疮病毒/分离鉴定/聚合酶链反应/动物回归试验Key words
Orf virus/isolation and identification/PCR/animal regression test分类
农业科技引用本文复制引用
周铭,岳进华,刘鹤媛,高洋,李雯丽,杨启明,陈德坤..陕西榆林某羊场羊口疮病毒的分离鉴定[J].动物医学进展,2016,37(9):31-34,4.基金项目
陕西省科技统筹创新工程科技计划项目 （）
