动物医学进展2016，Vol.37Issue(9)：38-42,5.
山豆根多糖对感染 PRRSV 小鼠脾脏细胞因子水平的影响
Effect of Sophora subprostrate Polysaccharide on Cytokine Secretion Levels in Spleen of Mice Infected with Porcinere Productive and Respiratory Syndrome Virus
摘要
Abstract
To investigate the effect of Sophora subprostrate polysaccharide(SSP)on the cytokine secretion level in splenic lymphocytes of mice infected with PRRSV,Kunming mice were set as target animals and 70 mice were divided into 7 groups randomly (A,B,C,D,E,F and G groups),ten mice each group with half male and half female.According to our preliminary experimental conditions for establishing the oxidative stress model,the animals in groups of D,E,F and G were treated with 1.0 mL virus stock by three ways of oral,intranasal adminstration and intraperitoneal injection on the day 1,day 2 and day 3.The animals in groups of A,B and C were treated with 1.0 mL of normal saline.The mice in groups of A and D were intra-peritoneally injected with normal saline at 0.2 mL per 10 g body weight on day 4,day 5 and day 6.The ani-mals in group B were treated with lipopolysaccharide(LPS)at dose of 5.0 mg/kg body weight by intraper-itoneal injection on day 4,day 5 and day 6.The animals in groups of C,E,F and G were treated with SSP at doses of 200,50,100,200 mg/kg body weight respectively on day 4,day 5 and day 6.On day 14,the animals were sacrificed and homogenates of murine spleen were prepared using physiological saline.ELISA method was used to determine the levels of TNF-α,IL-1β,IL-6,IL-8,IL-10,MCP-1 in the supernatant of homoge-nates.The levels of TNF-α,IL-1β,IL-6,IL-8,IL-10,MCP-1 significantly increased in the animals infected with PRRSV,SSP treatments at doses of 50 mg/kg and 100 mg/kg body weight significantly decreased a-bove cytokines in the infected mice on day 14 post infection.SSP played an important role in anti-inflamma-tion and anti-viral infection in PRRSV infected mice.关键词
山豆根多糖/猪繁殖与呼吸综合征病毒/小鼠/细胞因子Key words
Sophora subprostrate polysaccharide/Porcine reproductive and respiratory syndrome virus/mouse/cytokine分类
农业科技引用本文复制引用
李飞,韦英益,杨剑,陆晶莹,谭红连,胡庭俊..山豆根多糖对感染 PRRSV 小鼠脾脏细胞因子水平的影响[J].动物医学进展,2016,37(9):38-42,5.基金项目
国家自然科学基金项目 （）
