动物医学进展2016，Vol.37Issue(9)：58-62,5.
HPLC 法测定猪体内双氯芬酸的血药浓度
Determination of Diclofenac in Pig Plasma with HPLC
摘要
Abstract
In order to establish a HPLC method for the determination of plasma concentrations of diclofenac in piglets,hexane-isopropanol solution (90∶ 10,V/V)was used for extracting diclofenac of plasma.The plasma concentrations of diclofenac were measured by HPLC UV-Detector.The chromatography was car-ried on a C18 column,the mobile phase was composed of methanol-15 g/L acetic acid (20∶80,V/V),the detection wavelength was set at 276 nm for diclofenac,and analysis based on the external standard method. Under the condition of this test,diclofenac in plasma was well separated,and no interference from plasma matrix was observed.The linear ranges for diclofenac were 0.025 μg/mL-20.0 μg/mL(R2 =1),the lowest limit of quantitation was 0.05 μg/mL,and the ingredients showed good relationships between the peak area and the concentration.The inter-day and intra-day RSD was less than 4% and 6%.The recovery was be-tween 85% and 97%.The method could be used for the determination the diclofenac levels of piglets,and applied to study on the pharmacokinetics and bioavailability researches of the preparation containing di-clofenac sodium.关键词
高效液相色谱/双氯芬酸/血药浓度/仔猪Key words
HPLC/diclofenac/plasma drug concentration/piglet分类
农业科技引用本文复制引用
李宇琛,陈鸿雨,吴天兴,陆小松,韩孝欣,刘芳,王东亮,李龙飞,卜仕金..HPLC 法测定猪体内双氯芬酸的血药浓度[J].动物医学进展,2016,37(9):58-62,5.基金项目
江苏高校优势学科建设工程资助项目（PAPD）；江苏省普通高校研究生科研创新计划项目（SJLX15-0675）；扬州大学“新世纪人才工程”资助项目 （）
