动物医学进展2016，Vol.37Issue(9)：79-83,5.
黄芪多糖对小鼠卵母细胞体外成熟的影响
Effects of Astragalus Polysaccharides on in Vitro Maturation of Mouse Oocytes
摘要
Abstract
In order to explore the effect of Astragalus polysaccharides(APS)in the process of in vitro matu-ration (IVM)on mouse oocytes,immature mouse oocytes were collected from 6-8 week old mice and cul-tured in vitro with APS(0.1 mg/mL,1.0 mg/mL,10 mg/mL),and the rates of germinal vesicle break-down (GVBD)and first polar body (PB1)elimination of oocytes were counted by statistics.The mitochon-drial membrane potential in each group was detected.Simultaneously,the mouse oocytes were cultured with methylglyoxal (MG)and APS,and the production of ROS (reactive oxygen species)was detection.The re-sults showed that addition of APS had no significant effects on mouse oocytes'maturation,including the rates of GVBD and PBE1.The result of addition of APS and MG for coculture of oocytes showed that the rate of PB1 elimination was significantly reduced in the group MG+10 mg/mL APS,and the ROS contents of oocyts increased significantly,but had no significant effect on GVBD rate.关键词
小鼠卵母细胞/黄芪多糖/体外培养Key words
mouse oocyte/Astragalus polysaccharide/in vitro maturation分类
农业科技引用本文复制引用
闫学花,王丽丽,张锐,文杨,李科瑛,杨萌,雷安民,张为民..黄芪多糖对小鼠卵母细胞体外成熟的影响[J].动物医学进展,2016,37(9):79-83,5.基金项目
国家自然科学基金项目 （）
