南方医科大学学报2016，Vol.36Issue(9)：1169-1174,6.DOI:10.3969/j.issn.1673-4254.2016.09.01
缺氧对大鼠药物转运体MDR1、MRP2表达的影响
Effect of hypoxia on expressions of MDR1 and MRP2 in rats
摘要
Abstract
Objective To study the changes in the physiological parameters and gene expression of two drug efflux transporters MDR1 and MRP2 in the small intestine, liver and kidney of rats exposed to acute hypoxia. Methods Eighteen Wistar rats were randomly divided into control group, hypoxia for 24 h group and hypoxia for 72 h group. Blood samples were obtained from the abdominal aorta of the rats after the exposure for analyzing the physiological indexes. The mRNA expressions of MDR1 and MRP2 were determined using Real-Time PCR, and their protein expressions were detected with enzyme-linked immunosorbent assay (ELISA). Results The physiological parameters of the rats in hypoxia group were significantly changed compared with those in the control group. The expressions of MDR1 and MRP2 mRNA and proteins in the small intestine, liver and kidney were significantly increased in rats with hypoxic exposure than in the control rats (P<0.05 or 0.01). As the hypoxic exposure prolonged, the two transporters showed different patterns of variation in different tissues. Conclusion Acute hypoxia affects the physiological parameters and expression levels of MDR1 and MRP2, thus causing changes in the metabolism of the substrates of the transporters. These changes may play an important role in the pharmacokinetics of drugs at a high altitude.关键词
缺氧/生理指标/MDR1/MRP2Key words
hypoxia/physiological indexes/MDR1/MRP2引用本文复制引用
罗冰峰,尹强,王荣,李文斌,鹿辉,贾正平..缺氧对大鼠药物转运体MDR1、MRP2表达的影响[J].南方医科大学学报,2016,36(9):1169-1174,6.基金项目
国家科技重大专项（2008ZXJ09014-010）；国家自然科学基金（81403004，81401552）；全军后勤科研“十二五”重大项目（AWS14L0005）；全军医学科研“十二五”重点项目（BWS12J012） Supported by National Natural Science Foundation of China (81403004,81401552) （81403004,81401552）
