南方医科大学学报2016，Vol.36Issue(9)：1181-1185,5.DOI:10.3969/j.issn.1673-4254.2016.09.03
孕鼠交通性污染物暴露对其子代雄性睾丸DNA甲基化水平的影响
Effects of maternal exposure to vehicle exhaust on the reproductive system and DNA methylation in male offspring mice
摘要
Abstract
Objective To explore the effect of exposure to vehicle exhaust in pregnant mice on the reproductive function and DNA methylation in male offspring mice. Methods Twenty pregnant mice were randomized into control group and vehicle exhaust exposure group (n=10) and exposed to routine laboratory condition and to vehicle exhaust for 10 consecutive days (8 h per day) in a tunnel with a heavy traffic, where the concentrations of TSP, PM10, PM2.5, SO2 and NOX and the decibel of noise were measured. The offspring mice were raised till reaching maturity, and the epididymides of the male mice were collected to test the weight coefficients, DNA methylation level, and mRNA levels of Aldh7a1 and Rpe. Results The body weight and the weight coefficients of the epididymides and testes differed significantly between the exposure group and the control group (P>0.05). The concentrations of TSP, PM2.5, PM10 and NOx and the decibel of noise were significantly higher in the traffic environment and the control environment (P<0.05). Reduced representation bisulphite sequencing (RRBS) and Gene ontology (GO) showed that 58 genes had significantly different methylation levels between the two groups, mostly relating to the process of spermatogenesis (P<0.05). Compared with the control group, Aldh7a1 and Rpe mRNA expressions in the testes were down-regulated significantly in the exposure group (P<0.05). Conclusion Exposure of pregnant mice to vehicle exhaust causes damages of the reproductive function in the male offspring mice.关键词
交通性污染物/孕期暴露/雄性子代/生殖系统/DNA甲基化Key words
vehicle exhaust/exposure, pregnancy/offspring mice/reproductive system/DNA methylation引用本文复制引用
唐旭,夏茵茵,唐靖媛,代华,刘星灿,程淑群,孟盼,张瑞源..孕鼠交通性污染物暴露对其子代雄性睾丸DNA甲基化水平的影响[J].南方医科大学学报,2016,36(9):1181-1185,5.基金项目
国家自然科学基金（81502777）；重庆市科委自然科学基金计划资助项目（cstc2015jcyjA10032）；重庆市教委科学技术研究项目（KJ1400231）；重庆市渝中区科技计划项目（20130126）；重庆市卫生计生委医学科研项目（2015MSXM219） Supported by National Natural Science Foundation of China (81502777) （81502777）
