南方医科大学学报2016，Vol.36Issue(9)：1192-1197,6.DOI:10.3969/j.issn.1673-4254.2016.09.05
慢性砷暴露对小鼠脑和血清代谢组学的影响
Effect of chronic arsenic exposure on mouse brain tissue and serum metabolomics
摘要
Abstract
Objective To observe the effect of chronic arsenic exposure on cerebral cortex and serum metabolics of mice and explore the mechanism of arsenic neurotoxicity. Methods Twelve 3-week-old male C57BL/6J mice were randomly assigned into exposure group and control group and exposed to sodium arsenite (50 mg/L) via drinking water and deionized water for 12 weeks, respectively. After the exposure, arsenic level in the cerebrum was determined by hydride generation-atomic fluorescence spectrometry. The metabolites in the cerebral cortex and serum were determined using gas chromatography-mass spectrometry (GC/MS) analysis. Principal component analysis (PCA) was used to analyze the difference of the metabolites between the exposure and the control groups. Online tools for analyzing metabolic pathways were used to identify the related metabolites pathways. Results Arsenic content in the brain of exposure group was significantly higher than that in the control group (P<0.05). The mice exposed to arsenic had a higher level of citric acid, phenylalanine, tyrosine, histidine and lysine in the cerebral cortex (P<0.05). Serum levels of serine, glycine, proline, aspartate and glutamate were significantly higher whileα-ketoglutaric acid level was significantly lower in the exposure group than in the control group (P<0.05). PCA analysis showed a significant difference in cerebral cortex and serum metabolites between the two groups. Conclusion Chronic arsenic exposure may affect the function of the central nervous system by interfering with amino acid metabolism and tricarboxylic acid cycle, which may be one of the mechanisms of arsenic neurotoxicity.关键词
代谢组学/砷暴露/神经毒性/主成分分析法Key words
metabolomics/arsenic exposure/neurotoxicity/principal component analysis引用本文复制引用
代华,夏茵茵,Ting-Li Han,Philip N. Baker,唐旭,张瑞源,杜航,蔡同建,程淑群..慢性砷暴露对小鼠脑和血清代谢组学的影响[J].南方医科大学学报,2016,36(9):1192-1197,6.基金项目
国家自然科学基金（81502777）；重庆市科委自然科学基金（cstc2012jjA10070；cstc2015jcyjA10032）；重庆市教委科学技术研究项目（KJ1400231）；重庆市渝中区科技计划项目（20130126）；重庆市卫生计生委医学科研项目（2015MSXM219） Supported by National Natural Science Foundation of China (81502777) （81502777）
