南方医科大学学报2016，Vol.36Issue(9)：1204-1208,5.DOI:10.3969/j.issn.1673-4254.2016.09.07
类风湿关节炎患者血清及滑液中DNase I活性检测及与炎症的相关性
Correlation of DNase I in serum and synovial fluid with inflammatory activity in patients with rheumatoid arthritis
摘要
Abstract
Objective To investigate the potential role of deoxyribonuclease I (DNase I) in the pathogenesis of rheumatoid arthritis (RA). Methods DNase I activity was measured by radial enzyme-diffusion method in serum samples from 83 RA patients and 60 healthy volunteers and in the synovial fluid (SF) from 27 RA patients and 38 patients with other inflammatory arthritis. SF cfDNA level was measured with Pico Green Kit, and the correlation among DNase I activity, cfDNA level and clinical parameters of RA patients was analyzed. Results Serum DNase I activity was significantly lower in RA patients than in the healthy control subjects (0.3065±0.1436 vs 0.4289±0.1976 U/mL, P<0.001), and was negatively correlated with ESR (r=-0.2862, P=0.0122), CRP (r=-0.2790, P=0.0184) and neutrophil cell counts (r=-0.287, P=0.011). SF DNase I activity was almost negative in patients with RA, ankylosing spondylitis (AS) and gouty arthritis (GA). SF cfDNA level in RA patients was significantly higher than that in patients with osteoarthritis (100.81 ± 142.98 vs 18.98 ± 31.40μg/mL, P=0.002), but similar to that in patients with AS (45.85 ± 47.67μg/mL, P=0.428) and GA (162.95 ± 97.49μg/mL, P=0.132). In patients with inflammatory arthritis, SF cfDNA level was positively correlated with ESR (r=0.4106, P=0.0116) and CRP (r=0.5747, P=0.0002). Conclusion Impairment of DNase I activity may be responsible for the enhanced NETs generation and plays a role in the pathogenesis of RA.关键词
关节炎,类风湿/脱氧核糖核酸酶Ⅰ/DNAKey words
arthritis, rheumatoid/deoxyribonuclease I/DNA引用本文复制引用
许夏雨,杨文芳,张思功,赵琴,梁丽君,王鑫,沈海丽..类风湿关节炎患者血清及滑液中DNase I活性检测及与炎症的相关性[J].南方医科大学学报,2016,36(9):1204-1208,5.基金项目
国家自然科学基金（81560267）；甘肃省科技支撑计划-社会发展类项目（144FKCA060）；甘肃省卫生行业科研计划管理项目（GWGL2014-47）；亚太风湿病学会研究基金2015 Supported by National Natural Science Foundation of China (81560267) （81560267）
