南方医科大学学报2016，Vol.36Issue(9)：1265-1270,6.DOI:10.3969/j.issn.1673-4254.2016.09.18
TRPM8通过cAMP-PKA/UCP4信号调控氧糖剥夺/复糖复氧诱导的神经元凋亡
TRPM8 mediates PC-12 neuronal cell apoptosis induced by oxygen-glucose deprivation through cAMP-PKA/UCP4 signaling
李宏维 1周斌 1张海鸿1
作者信息
- 1. 兰州大学第二医院甘肃省骨关节疾病重点实验室，甘肃 兰州 730000
- 折叠
摘要
Abstract
Objective To explore the molecular mechanism responsible for apoptosis of PC-12 neuronal cells induced by oxygen-glucose deprivation (OGD). Methods PC12 cells were exposed to OGD for 24 h to simulate ischemia-reperfusion injury. Flow cytometry was employed detect the cell apoptosis, and the expresions of TRPM8, UCP4, cAMP and PKA in the exposed cells were detected with RT-PCR and Western blotting. The changes in the expressions of Bax, Bcl-2, cAMP, PKA and UCP4 proteins were detected in the exposed cells in resposne to inhibition of TRPM8 and cAMP-PKA signal or over-expression of UCP4. Results OGD for 24 induced obvious apoptosis in PC-12 cells and caused TRPM8 over-expression and inhibition of UCP4 and cAMP-PKA signaling. Inhibiting TRPM8 expression reduced the cell apoptosis and up-regulated cAMP, p-PKA and UCP4 in the cells exposed to OGD. In cells exposed to OGD, inhibition of TRPM8 and cAMP-PKA signaling suppressed the expressio of UCP4 and increased the cell apoptosis. Conclusion TRPM8 mediates OGD-induced PC12 cell apoptosis through cAMP-PKA/UCP4 signaling.关键词
PC12细胞/氧糖剥夺/复糖复氧/TRPM8/cAMP-PKA/UCP4/凋亡Key words
PC12 cells/oxygen-glucose deprivation/TRPM8/cAMP-PKA/UCP4/apoptosis引用本文复制引用
李宏维,周斌,张海鸿..TRPM8通过cAMP-PKA/UCP4信号调控氧糖剥夺/复糖复氧诱导的神经元凋亡[J].南方医科大学学报,2016,36(9):1265-1270,6.
