南方医科大学学报2016，Vol.36Issue(9)：1286-1290,1294,6.DOI:10.3969/j.issn.1673-4254.2016.09.22
丙泊酚下调水通道蛋白3和基质金属蛋白酶-9表达抑制人肺癌A549细胞的侵袭力
Propofol suppresses invasion of human lung cancer A549 cells by down-regulating aquaporin-3 and matrix metalloproteinase-9
摘要
Abstract
Objective To investigate the effect of propofol on cell invasion and expressions of aquaporin-3 (APQ-3) and matrix metalloproteinase-9 (MMP-9) in human lung adenocarcinoma cancer A549 cells. Method A549 cells were treated with propofol at the concentrations of 25, 50, and 100 μmol/L for 12 or 24 h. RT-PCR was used to detect the effect of propofol on AQP-3 mRNA level in A549 cells, and the effects of propofol treatments for 24 h on AQP-3 and MMP-9 protein expression and the invasive ability of A549 cells were assessed with Western blotting and Transwell assay, respectively. Results Compared with the control cells, the cells treated with 25, 50, and 100 μmol/L propofol showed a obvious inhibition of AQP-3 mRNA expression, with inhibition rates ranging from 0.19 to 0.65 in cells with a 12-h treatment and from 0.13 to 0.41 in cells treated for 24 h;100μmol/L propofol treatment for 24 h produced the strongest inhibitory effect (0.13 ± 0.035, P<0.05). AQP-3 protein expression in cells treated with 25, 50, and 100μmol/L propofol for 24 h (0.91 ± 0.009, 0.60 ± 0.020, and 0.57 ± 0.006, respectively) and MMP-9 protein expression in cells treated with 50 and 100 μmol/L propofol for 24 h (0.65 ± 0.006 and 0.46 ± 0.021, respectively) were significantly lower than those in the control cells (P<0.05). Treatment with 25, 50, and 100μmol/L propofol for 24 significantly lowered the number of invading cells (122.55 ± 17.20, 96.33 ± 5.82, and 74.33 ± 2.85, respectively) compared with the control group (199.33±23.88, P<0.05). Conclusion Treatment with 50 and 100μmol/L propofol inhibits cell invasion by down-regulating the expression of AQP-3 and MMP-9 in A549 cells.关键词
丙泊酚/水通道蛋白3/基质金属蛋白酶9/A549/侵袭力Key words
propofol/aquaporin-3/matrix metalloproteinase-9/A549/invasion引用本文复制引用
叶慧瑾,白建杰,郭培培,汪威,林春水..丙泊酚下调水通道蛋白3和基质金属蛋白酶-9表达抑制人肺癌A549细胞的侵袭力[J].南方医科大学学报,2016,36(9):1286-1290,1294,6.基金项目
广东省科技计划项目（2012A030400014）；广州市科技计划项目 （）
