口腔疾病防治2016，Vol.24Issue(9)：511-514,4.DOI:10.12016/j.issn.2096-1456.2016.09.003
牛磺酸上调基因1在舌鳞状细胞癌中的作用研究
Study on the role of long non-coding RNA TUG1 in tongue squamous cell carcinoma
摘要
Abstract
Objective To study the expression of LncRNA TUG1 in tongue squamous cell carcinoma (TSCC), and explore its function in the development of TSCC. Methods Fresh samples of TSCC tissues and corresponding adjacent normal tongue tissues were obtained from 19 cases, and the expressions of TUG1were detected by Q⁃PCR. The expres⁃sion of TUG1 in CAL27 cell line were down⁃regulated by siRNA technology, and the cell proliferation ability after trans⁃fection was analyzed by MTT assay. The expressions of iNOS gene were rated by Q⁃PCR. Results Result of Q⁃PCR re⁃vealed the expressions of LncRNA TUG1 were up⁃regulated in TSCC tissues compared with the adjacent normal tongue tissues (P<0.001). Cell proliferation ability was inhibited after down⁃regulation of TUG1 by siRNA technology, and the inhibition rates of 24/48/72 hour were 14.16%. 16.96% and 21.40% respectively. The relative expression of iNOS in blank/control/experimental groups were 1.000 ± 0.034, 0.974 ± 0.045 and 0.729 ± 0.039 respectively, and iNOS was down⁃regulated significantly in the experimental group (P = 0.002). Conclusion LncRNA TUG1 may have functions as a cancerogenic substance in TSCC via pormoting the expression of iNOS.关键词
舌/鳞状细胞癌/长链非编码RNA/牛磺酸上调基因1/诱导型一氧化氮合酶Key words
Tongue/Squamous cell carcinoma/Long non-coding RNA/Taurine upregulated gene 1/Induc-ible nitric oxide synthase分类
医药卫生引用本文复制引用
李志强,邹瑞,欧阳可雄,白植宝,艾伟健..牛磺酸上调基因1在舌鳞状细胞癌中的作用研究[J].口腔疾病防治,2016,24(9):511-514,4.基金项目
广东省医学科研基金（A2014106）；广东省科技计划项目 （）
