口腔疾病防治2016，Vol.24Issue(9)：519-523,5.DOI:10.12016/j.issn.2096-1456.2016.09.005
不同感染程度根管内微生物群落宏基因分析的对比研究
Comparison of the bacterial community structures in root canals with different severity of infection by metage-nomic analysis
摘要
Abstract
Objective To compare the intraradicular bacterial community structures in under⁃filling teeth with or withoutperiapical infection. Methods The intraradicularbiofilm samples were collected from under⁃filling teeth with periapicalinfection (apical periodontitis group, 3 teeth) and underfilling teeth withoutperiapical infection (non⁃apical periodontitis group, 2 teeth). The V3, V6 hypervariable regions of bacterial 16S rRNA genes were amplified, and the high⁃throughput sequencingwasperformed. Thecomposition and structure characteristic of intraradicular microbiota were revealed by bioinformatics analysis. Results The microbiotain apical periodontitis group comprised of Firmicutes, Pro⁃teobacteria, Actinobacteriad and others, total 22 phyla. While the microbiotain non⁃apical periodontitis group com⁃prised of Firmicutes, Proteobacteria, Actinobacteria and others, total 8 phyla. The complexity of the microbiotain apical periodontitis groupwas significantly higher than without apical periodontitis group, but both groups comprised thesimi⁃lar most representivephyla. Conclusion The diversity of intraradicular bacterial community in underfillingteeth with apical periodontitis was higher than those in non⁃apical periodontitis.关键词
根尖周病变/宏基因分析/生物膜/微生物群落/多样性Key words
Periapical periodontitis/Metagenomic analysis/Biofilm/Microbiota/Diversity分类
医药卫生引用本文复制引用
周琦,李军心,钟波,张晓洁,江双风..不同感染程度根管内微生物群落宏基因分析的对比研究[J].口腔疾病防治,2016,24(9):519-523,5.基金项目
深圳市科技创新委员会知识创新计划 （）
