临床与病理杂志2016，Vol.36Issue(8)：1079-1086,8.DOI:10.3978/j.issn.2095-6959.2016.08.007
Carba NP试验及Carba NP-direct试验检测产碳青霉烯酶肠杆菌的临床意义
The clinical significance of Carba NP test and Carba NP-direct test in the detection of carbapenemase producing Enterobacteriaceae strains
胡仁静 1严子禾 1韩志君 1胡锡池1
作者信息
- 1. 南京医科大学附属无锡市第二人民医院检验科，江苏 无锡 214002
- 折叠
摘要
Abstract
Objective: Carba NP test and CNPt-direct were evaluated for the carbapenemase detection of carbapenem-resistantEnterobacteriaceae.Methods: We collected 112 carbapenem-resistantEnterobacteriaceae and 100 carbapenem-susceptibleEnterobacteriaceae in this study. They were isolated between January 1, 2013 and December 31, 2015 from various clinical samples and surveillance samples from the Second People’s Hospital of Wuxi Affiliated to Nanjing Medical University (Wuxi, China). Carbapenemase genes detectionby PCR, Modified Hodge test (MHT), Carba NP test (CNPt) and Carba NP-direct test (CNPt-d) were performed.Results: 92/135 carbapenem-resistantEnterobacteriaceae isolates carried blaKPC-2; 8 isolates carried blaNDM-1; 1 isolates carried blaVIM-2; 1 isolates carried blaIMP-4; non-isolates carried class D carbapenemase; 10 isolates carried non-carbapenemase genes. Carbapenemases genes PCR detection was regarded as a gold standard, the sensitivity and speciifcity of Carba NP test and CNPt-direct were 100%; the sensitivity and speciifcity of Modiifed Hodge test was 96% and 98%. Both Carba NP test and CNPt-direct can be done within 2 h.Conclusion:CNPt and the CNPt-d are accurate for rapid carbapenemase detection. CNPt-direct is cheaper, faster and more deifnite, CNPt-d is very promising, for epidemiological and infection control purpose.关键词
肠杆菌科细菌/Carba NP试验/Carba NP-direct试验/碳青霉烯酶Key words
Enterobacteriaceae/Carba NP test/Carba NP-direct test/carbapenemase引用本文复制引用
胡仁静,严子禾,韩志君,胡锡池..Carba NP试验及Carba NP-direct试验检测产碳青霉烯酶肠杆菌的临床意义[J].临床与病理杂志,2016,36(8):1079-1086,8.
