临床与病理杂志2016，Vol.36Issue(8)：1123-1128,6.DOI:10.3978/j.issn.2095-6959.2016.08.015
ATF4对皮肤癌细胞增殖的影响及相关机制
Effects and mechanisms of ATF4 on skin cancer cell proliferation
摘要
Abstract
Objective: To investigate the effects and mechanisms of knockdown/over-expression of ATF4 on the skin cancer cell proliferation.Methods: hTe expression of ATF4 in different types of skin cancer cells was measured by Western blot. Atfer knockdown or over-expression of ATF4 in A431 cells, the proliferation and cell cycle distribution were measured by CCK-8 assay, colony formation assay or lfow cytometry. hTe protein levels of some cell cycle regulators, such as cyclin D1, cyclin E, P21 and p-Rb/Rb were determined by Western blot. Results: ATF4 was highly expressed in all 3 types of skin cancer cells (P<0.05). ATF4 knockdown repressedthe cell cycle entrance into S-phase from G0/G1 phase followed by a lower cell proliferation rate. Over-expression of ATF4 increased A431 cell proliferation and facilitated cell cycle progression (P<0.05). Furthermore, the protein levels of cyclin D1, cyclin E and p-Rb were significantly decreased after ATF4 knockdown, but the expression of P21 was increased. Over-expression of ATF4 increased the protein levels of cyclin D1, cyclin E and p-Rb, but decreased the P21 expression (P<0.05).Conclusion: ATF4 promotes human squamous cell carcinoma, and its potential mechanisms may be related to promotion of cell cycle transition and expression of cell cycle regulators, suggesting that ATF4 may be a potential new target of treating skin cancer.关键词
ATF4/皮肤癌/细胞增殖/细胞周期Key words
ATF4/human skin cancer/proliferation/cell cycle引用本文复制引用
陈娟,王翼..ATF4对皮肤癌细胞增殖的影响及相关机制[J].临床与病理杂志,2016,36(8):1123-1128,6.基金项目
Foundation item)陕西省卫生厅中医药科技发展计划项目(LZ14092)。This work was supported by Shaanxi Province Health Development of Science and Technology Plan Projects of Chinese Medicine (LZ14092), P. R. China （LZ14092）
