广西科学院学报2016，Vol.32Issue(3)：190-208,19.DOI:10.13657/j.cnki.gxkxyxb.20160517.005
古菌、细菌和真核生物纤维素酶的整体进化关系
Overall Evolutionary Relationship of Cellulases from Archaea,Bacteria and Eukaryota
摘要
Abstract
[Objective]Cellulases have wide applications in biofuel,food,paper,pharmaceutical, and textile industries.Many organisms produce cellulases,therefore there is a very complicat-ed evolutionary relationship for cellulases.Such a relationship is further tangled by the ef-forts that manipulate cellulase structures and express cellulase genes in different species to improve their activity and suitability.So far,no large-scale phylogenetic studies have been conducted to analyze the overall evolutionary relationship of cellulases.Yet no statistical studies have been conducted to exhaustively analyze variances of cellulases and their parti-tioned variances along taxonomic lineage from superkingdom to organism.[Methods]To fill these knowledge gaps,2 416 cellulases,including 116,1 744 and 556 from archaea,bacteria and eukaryota,were studied using statistical and phylogenetic analyses.Statistical analysis was conducted by computing the average pairwise p-distance for each phylum,class,order, family and genus,and by further partitioning the variance into inter-clan variance and intra-clan variance along taxonomic lineage.Large-scale phylogenetic analysis was performed through the phylogenetic tree of cellulases.[Results]The average pairwise p-distance of ami-no acid sequences varied greatly in the cellulases from different phylum,class,order,family and genus.The inter-clan variance and intra-clan variance varied also greatly along taxonomic lineage but a light pattern could be found in eukaryota.For large-scale phylogenetic analyses, most cellulases underwent different paths and bifurcations,and formed intertwined clusters, where many meaningful hints can be tracked.[Conclusion]This large-scale study provides the insights on overall evolutionary relationship of cellulases from both statistical and phylogenetic viewpoints.As additional information,the statis-tical analysis not only enriches the phylogenetic analysis,but also suggests how to biotechnologically manipulate cellulases with desired quali-ties,and how the evolution of cellulases are involved with vertical inheritance,horizontal gene transfer,multiple times and multiple clans.关键词
纤维素酶/进化/种属间差异/种属内差异/系统发育Key words
cellulase/evolution/inter-clan variance/intra-clan variance/phylogenetics分类
生物科学引用本文复制引用
严少敏,吴光..古菌、细菌和真核生物纤维素酶的整体进化关系[J].广西科学院学报,2016,32(3):190-208,19.基金项目
国家自然科学基金项目(21466007,31400079,31460296,31560315),广西自然科学基金重点项目(2013GXNSFDA019007),广西国际科技成果引进与合作研究项目(1347004-1)和广西人才小高地建设专项基金项目资助。 （21466007,31400079,31460296,31560315）
