激光生物学报2016，Vol.25Issue(4)：379-385,7.DOI:10.3969/j.issn.1007-7146.2016.04.015
水霉拮抗放线菌的分离、筛选与鉴定
Isolation,Screening and Identification of Antagonistic Actinomycetes against Saprolegniasis
摘要
Abstract
Objective:Isolation,screening and identification of antagonistic actinomycetes against Saprolegniasis from the sediment in healthy aquaculture water of pearl.Methods:Using double resistant screen plate containing nalidixic acid and cycloheximide,dilution and spread plate method was applied to isolate actinomycetes.Taking pathogenic sapro-legnia isolated from the fish eggs of Pelteobagrus fulvidraco and Triploid crucian carp as test bacteria,Antimicrobial ac-tivity against saprolegnia and its stability were assessed using agar diffusion method.The isolates with high antimicrobial activity were identified by morphology and 16S rDNA gene sequence analysis.Results:3 strains of antagonistic actino-mycetes,named as QF1,DNC17,QHV2,were screened from the 27 actinomycetes which were isolated from the sedi-ment samples.Among the 3 isolates,QHV2 strain had the best antibacterial activity and stability.Based on morphology and 16S rDNA gene sequencing,QF1,DNC17 and QHV2 strains all belonged to the genus Streptomyces,and were iden-tified as Streptomyces diastatochromogenes,Streptomyces variabilis and Streptomyces collinus respectively.Conclusion:Taken together,the 3 isolated actinomycetes have higher antagonistic activity against saprolegnia,suggesting that they have potential value of the medicine development for the preventing and controlling of Saprolegniasis.关键词
水霉/拮抗菌/放线菌/分离与筛选/分子鉴定Key words
Saprolegnia/antagonistic bacteria/actinomycetes/isolation and screening/molecular identification分类
农业科技引用本文复制引用
陈飘,罗玉双,张永丽,杨敏,张紫根..水霉拮抗放线菌的分离、筛选与鉴定[J].激光生物学报,2016,25(4):379-385,7.基金项目
湖南省高校创新平台开放基金项目（12K121）；微生物分子生物学湖南省重点实验室开放基金项目（2013-02）；湖南省大学生研究性学习和创新性实验计划项目（湘教通【2014】248号）；湖南文理学院芙蓉学院研究性学习和创新性实验计划项目 （）
